PMID- 18709192
OWN - NLM
STAT- MEDLINE
DCOM- 20081205
LR  - 20080818
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 133
IP  - 9
DP  - 2008 Sep
TI  - An electrochemical sensor for pesticide assays based on carbon nanotube-enhanced 
      acetycholinesterase activity.
PG  - 1182-6
LID - 10.1039/b805334k [doi]
AB  - There has been urgent demand for rapid, sensitive and cost-effective pesticide 
      assay technologies due to the global attention of environmental and food-safety 
      problems. Acetycholinesterase (AChE)-based electrochemical sensors have attracted 
      significant interest toward this goal. In this contribution, we introduced 
      multiwalled carbon nanotubes (MWNTs) into our sensor design, where they played 
      dual enhancement roles; first is that MWNTs loaded on glassy carbon (GC) 
      electrodes significantly increase surface areas, facilitating the electrochemical 
      polymerization of prussian blue (PB), a redox mediator for the electrochemical 
      oxidation of the enzymatic product, thiocholine (TCh). Second, MWNTs enhance the 
      enzymatic activity of AChE, as manifested by the decreased Michaelis-Menten 
      constant (K(m)). As a result of these two important enhancement factors offered 
      by MWNTs, our electrochemical pesticide sensor exhibited rapid response and high 
      sensitivity toward the detection of a series of pesticides. Moreover, we 
      demonstrated that this sensor was stable, reproducible and selective enough for 
      detection in real samples.
FAU - Chen, Haode
AU  - Chen H
AD  - College of Life Science and Biotechnology, Shanghai Jiao Tong University, 
      Shanghai 200240, China.
FAU - Zuo, Xiaolei
AU  - Zuo X
FAU - Su, Shao
AU  - Su S
FAU - Tang, Zhuzhao
AU  - Tang Z
FAU - Wu, Aibo
AU  - Wu A
FAU - Song, Shiping
AU  - Song S
FAU - Zhang, Dabing
AU  - Zhang D
FAU - Fan, Chunhai
AU  - Fan C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080721
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Environmental Pollutants)
RN  - 0 (Nanotubes, Carbon)
RN  - 0 (Pesticides)
RN  - EC 3.1.1.7 (Acetylcholinesterase)
SB  - IM
MH  - Acetylcholinesterase
MH  - Electrochemistry/instrumentation/methods
MH  - Environmental Monitoring/instrumentation/methods
MH  - Environmental Pollutants/*analysis
MH  - Equipment Design
MH  - Nanotubes, Carbon
MH  - Pesticides/*analysis
EDAT- 2008/08/19 09:00
MHDA- 2008/12/17 09:00
CRDT- 2008/08/19 09:00
PHST- 2008/08/19 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/08/19 09:00 [entrez]
AID - 10.1039/b805334k [doi]
PST - ppublish
SO  - Analyst. 2008 Sep;133(9):1182-6. doi: 10.1039/b805334k. Epub 2008 Jul 21.