PMID- 18710945
OWN - NLM
STAT- MEDLINE
DCOM- 20081024
LR  - 20240415
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 28
IP  - 20
DP  - 2008 Oct
TI  - Regulation of the endosomal SNARE protein syntaxin 7 by colony-stimulating factor 
      1 in macrophages.
PG  - 6149-59
LID - 10.1128/MCB.00220-08 [doi]
AB  - Colony-stimulating factor 1 (CSF-1) is the main growth factor controlling the 
      development of macrophages from myeloid progenitor cells. However, CSF-1 also 
      regulates some of the key effector functions of macrophages (e.g., phagocytosis 
      and cytokine secretion). The endosomal SNARE protein syntaxin 7 (Stx7) regulates 
      vesicle trafficking events involved in phagocytosis and cytokine secretion. 
      Therefore, we investigated the ability of CSF-1 to regulate Stx7. CSF-1 
      upregulated Stx7 expression in primary mouse macrophages; it also upregulated 
      expression of its SNARE partners Vti1b and VAMP8 but not Stx8. Additionally, 
      CSF-1 induced the rapid serine phosphorylation of Stx7 and enhanced its binding 
      to Vti1b, Stx8, and VAMP8. Bioinformatics analysis and results from experiments 
      with kinase inhibitors suggested the CSF-1-induced phosphorylation of Stx7 was 
      mediated by protein kinase C and Akt in response to phosphatidylinositol 3-kinase 
      activation. Based on mutagenesis studies, CSF-1 appeared to increase the binding 
      of Stx7 to its SNARE partners by inducing the phosphorylation of serine residues 
      in the Habc domain and/or "linker" region of Stx7. Thus, CSF-1 is a key regulator 
      of Stx7 expression and function in macrophages. Furthermore, the effects of CSF-1 
      on Stx7 may provide a mechanism for the regulation of macrophage effector 
      functions by CSF-1.
FAU - Achuthan, Adrian
AU  - Achuthan A
AD  - Department of Medicine, The University of Melbourne, Royal Melbourne Hospital, 
      Parkville, Victoria, Australia.
FAU - Masendycz, Paul
AU  - Masendycz P
FAU - Lopez, Jamie A
AU  - Lopez JA
FAU - Nguyen, Thao
AU  - Nguyen T
FAU - James, David E
AU  - James DE
FAU - Sweet, Matthew J
AU  - Sweet MJ
FAU - Hamilton, John A
AU  - Hamilton JA
FAU - Scholz, Glen M
AU  - Scholz GM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080818
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 0 (Qa-SNARE Proteins)
RN  - 17885-08-4 (Phosphoserine)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.13 (Protein Kinase C)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Computational Biology
MH  - Endosomes/drug effects/enzymology/*metabolism
MH  - Enzyme Activation/drug effects
MH  - Enzyme Inhibitors/pharmacology
MH  - Female
MH  - Macrophage Colony-Stimulating Factor/*pharmacology
MH  - Macrophages/*drug effects/enzymology/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Models, Biological
MH  - Molecular Sequence Data
MH  - Mutation/genetics
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Phosphoinositide-3 Kinase Inhibitors
MH  - Phosphorylation/drug effects
MH  - Phosphoserine/metabolism
MH  - Protein Binding/drug effects
MH  - Protein Kinase C/metabolism
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Qa-SNARE Proteins/chemistry/genetics/*metabolism
MH  - Up-Regulation/drug effects
PMC - PMC2577439
EDAT- 2008/08/20 09:00
MHDA- 2008/10/25 09:00
PMCR- 2009/04/01
CRDT- 2008/08/20 09:00
PHST- 2008/08/20 09:00 [pubmed]
PHST- 2008/10/25 09:00 [medline]
PHST- 2008/08/20 09:00 [entrez]
PHST- 2009/04/01 00:00 [pmc-release]
AID - MCB.00220-08 [pii]
AID - 0220-08 [pii]
AID - 10.1128/MCB.00220-08 [doi]
PST - ppublish
SO  - Mol Cell Biol. 2008 Oct;28(20):6149-59. doi: 10.1128/MCB.00220-08. Epub 2008 Aug 
      18.