PMID- 18712556
OWN - NLM
STAT- MEDLINE
DCOM- 20090306
LR  - 20211020
IS  - 1367-5435 (Print)
IS  - 1367-5435 (Linking)
VI  - 35
IP  - 11
DP  - 2008 Nov
TI  - Use of quantitative real-time PCR to monitor population dynamics of 
      ammonia-oxidizing bacteria in batch process.
PG  - 1339-44
LID - 10.1007/s10295-008-0416-8 [doi]
AB  - A quantitative real-time PCR (QPCR) assay with the TaqMan system was used to 
      quantify 16S rRNA genes of beta-proteobacterial ammonia-oxidizing bacteria (AOB) 
      in a batch nitrification bioreactor. Five different sets of primers, together 
      with a TaqMan probe, were used to quantify the 16S rRNA genes of 
      beta-proteobacterial AOB belonging to the Nitrosomonas europaea, Nitrosococcus 
      mobilis, Nitrosomonas nitrosa, and Nitrosomonas cryotolerans clusters, and the 
      genus Nitrosospira. We also used PCR followed by denaturing gradient gel 
      electrophoresis (DGGE), cloning, and sequencing of their 16S rRNA genes to 
      identify the AOB species. Seed sludge from an industrial wastewater treatment 
      process controlling high-strength nitrogen wastewater (500 mg/L NH4+-N) was used 
      as the inoculum for subsequent batch experiment. The Nitrosomonas nitrosa cluster 
      was the predominant AOB (2.3x10(5) copies/mL) in the start-up period of the batch 
      experiment. However, from the exponential growth period, the Nitrosomonas 
      europaea cluster was the most abundant AOB, and its 16S rRNA gene copy number 
      increased to 8.9x10(6) copies/mL. The competitive dominance between the two AOB 
      clusters is consistent with observed differences in ammonia tolerance and 
      substrate affinity. Analysis of the DGGE results indicated the presence of 
      Nitrosomonas europaea ATCC19718 and Nitrosomonas nitrosa Nm90, consistent with 
      the QPCR results.
FAU - Lim, Juntaek
AU  - Lim J
AD  - School of Environmental Science and Engineering, Pohang University of Science and 
      Technology, Pohang, Gyungbuk, 790-784, South Korea. jtwwb@postech.ac.kr
FAU - Lee, Seungyong
AU  - Lee S
FAU - Hwang, Seokhwan
AU  - Hwang S
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080820
PL  - Germany
TA  - J Ind Microbiol Biotechnol
JT  - Journal of industrial microbiology & biotechnology
JID - 9705544
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - 7664-41-7 (Ammonia)
SB  - IM
MH  - Ammonia/*metabolism
MH  - *Biodiversity
MH  - Bioreactors/microbiology
MH  - DNA Primers/genetics
MH  - DNA, Bacterial/genetics
MH  - DNA, Ribosomal/genetics
MH  - Nitrosomonadaceae/*genetics/isolation & purification/*metabolism
MH  - Polymerase Chain Reaction/*methods
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sewage/microbiology
EDAT- 2008/08/21 09:00
MHDA- 2009/03/07 09:00
CRDT- 2008/08/21 09:00
PHST- 2008/03/24 00:00 [received]
PHST- 2008/07/29 00:00 [accepted]
PHST- 2008/08/21 09:00 [pubmed]
PHST- 2009/03/07 09:00 [medline]
PHST- 2008/08/21 09:00 [entrez]
AID - 10.1007/s10295-008-0416-8 [doi]
PST - ppublish
SO  - J Ind Microbiol Biotechnol. 2008 Nov;35(11):1339-44. doi: 
      10.1007/s10295-008-0416-8. Epub 2008 Aug 20.