PMID- 18713300
OWN - NLM
STAT- MEDLINE
DCOM- 20090507
LR  - 20221207
IS  - 1440-1746 (Electronic)
IS  - 0815-9319 (Linking)
VI  - 24
IP  - 2
DP  - 2009 Feb
TI  - Polymorphism of human leptin receptor gene is associated with type 2 diabetic 
      patients complicated with non-alcoholic fatty liver disease in China.
PG  - 228-32
LID - 10.1111/j.1440-1746.2008.05544.x [doi]
AB  - BACKGROUND AND AIM: To investigate the relationship between human leptin receptor 
      (LEPR) gene G3057A polymorphism and type 2 diabetes mellitus (T2DM) patients 
      complicated with or without non-alcoholic fatty liver disease (NAFLD). METHODS: 
      Two hundred and sixteen cases of newly diagnosed T2DM patients (104 cases 
      complicated with NAFLD) and 108 cases of normal glucose tolerances (NGT) were 
      recruited. Hemi-nested polymerase chain reaction restriction fragment length 
      polymorphism (PCR-RFLP) and PCR-direct sequence analysis were conducted to detect 
      the polymorphism of LEPR G3057A variation. Plasma leptin levels were measured by 
      enzyme-linked immunosorbent assay kit. Plasma lipid and glucose metabolic 
      parameters were measured routinely. Liver ultrasound was carried out for all 
      subjects. RESULTS: T2DM patients complicated with NAFLD had higher plasma 
      insulin, leptin, triglycerides (TG) and low-density lipoprotein cholesterol 
      (LDL-C) levels than those without NAFLD and NGT subjects. The variant frequency 
      at nucleotide 3057 G-->A transversion was 76.0% in type 2 diabetic patients 
      complicated with NAFLD, which was also significantly higher than those without 
      NAFLD (62.1%) and NGT cases (53.2%). There was also significant difference in 
      genotype distribution between the three groups (chi(2) = 14.63, P < 0.01). 
      CONCLUSION: The polymorphism of LEPR gene 3057 probably contributes to the onset 
      of NAFLD by regulating lipid metabolism and affecting insulin sensitivity.
FAU - Lu, Hongyun
AU  - Lu H
AD  - Department of Endocrinology, the Fifth Affiliated Hospital of Sun Yat-sen 
      University, Zhuhai, Guangdong, China. luhongyun1120@yahoo.com.cn
FAU - Sun, Jiazhong
AU  - Sun J
FAU - Sun, Liao
AU  - Sun L
FAU - Shu, Xiaochun
AU  - Shu X
FAU - Xu, Yancheng
AU  - Xu Y
FAU - Xie, Danhong
AU  - Xie D
LA  - eng
PT  - Journal Article
DEP - 20080817
PL  - Australia
TA  - J Gastroenterol Hepatol
JT  - Journal of gastroenterology and hepatology
JID - 8607909
RN  - 0 (Blood Glucose)
RN  - 0 (Cholesterol, LDL)
RN  - 0 (Insulin)
RN  - 0 (LEPR protein, human)
RN  - 0 (Leptin)
RN  - 0 (Receptors, Leptin)
RN  - 0 (Triglycerides)
SB  - IM
CIN - J Gastroenterol Hepatol. 2009 Feb;24(2):173-5. PMID: 19215327
MH  - Aged
MH  - Asian People/*genetics
MH  - Blood Glucose/analysis
MH  - Case-Control Studies
MH  - China/epidemiology
MH  - Cholesterol, LDL/blood
MH  - DNA Mutational Analysis
MH  - Diabetes Complications/blood/ethnology/*genetics
MH  - Diabetes Mellitus, Type 2/blood/complications/ethnology/*genetics
MH  - Fatty Liver/blood/ethnology/*genetics
MH  - Female
MH  - Gene Frequency
MH  - Genetic Predisposition to Disease
MH  - Humans
MH  - Insulin/blood
MH  - Leptin/blood
MH  - Liver/chemistry/diagnostic imaging
MH  - Logistic Models
MH  - Male
MH  - Middle Aged
MH  - Phenotype
MH  - Polymerase Chain Reaction
MH  - *Polymorphism, Genetic
MH  - Receptors, Leptin/*genetics
MH  - Risk Assessment
MH  - Risk Factors
MH  - Triglycerides/blood
MH  - Ultrasonography
EDAT- 2008/08/21 09:00
MHDA- 2009/05/08 09:00
CRDT- 2008/08/21 09:00
PHST- 2008/08/21 09:00 [pubmed]
PHST- 2009/05/08 09:00 [medline]
PHST- 2008/08/21 09:00 [entrez]
AID - JGH5544 [pii]
AID - 10.1111/j.1440-1746.2008.05544.x [doi]
PST - ppublish
SO  - J Gastroenterol Hepatol. 2009 Feb;24(2):228-32. doi: 
      10.1111/j.1440-1746.2008.05544.x. Epub 2008 Aug 17.