PMID- 18713408
OWN - NLM
STAT- MEDLINE
DCOM- 20081103
LR  - 20221207
IS  - 1744-7909 (Electronic)
IS  - 1672-9072 (Linking)
VI  - 50
IP  - 6
DP  - 2008 Jun
TI  - A confocal technique applicable to studies of cellular pH-related signaling in 
      plants.
PG  - 682-90
LID - 10.1111/j.1744-7909.2008.00667.x [doi]
AB  - pH may act as a crucial signal in both animal and plant cells. It is very 
      difficult to monitor pH signals and this has largely hindered progress in the 
      investigation of pH signaling, particularly systematic pH signaling. Here, we 
      report the development of a confocal technique to monitor leaf apoplastic pH in 
      intact plants, which is particularly suitable for the studies on root to shoot 
      signaling. A variety of different pH indicators and plant species were tested. It 
      was found that different pH indicators, for example, 
      2',7'-Bis-(2-carboxyethyl)-5-(and-6)-carboxyfluoresce (BCECF), SNARF-4F 
      5-(and-6)-carboxylic acid (SNARF) and DM-NERF (NERF), were of different 
      properties, and to successfully monitor pH at a sub-cellular level, the 
      comparability between the pH indicator and plant species must be involved 
      according to their suitable pH range and loading characteristics. The loading 
      characteristics of different pH indicators differ with different plant species, 
      cell types and their developing stages. No matter what methods were adopted, 
      BCECF and SNARF could not be loaded specifically in the leaf apoplast in 
      sunflower, tomato, and Comelina communis L. In contrast, regardless of the 
      methods adopted, NERF could be loaded efficiently and specifically in the leaf 
      apoplast in C. communis, but not in other plants. In C. communis, the 
      determination coefficient for in vitro and in situ calibration of NERF was very 
      high, which was respectively 0.9951 and 0.9916, and therefore, the adoption of 
      NERF together with C. communis could construct an ideal experimental system that 
      is suitable for the investigation of pH systematic signaling. Ratio image 
      analysis demonstrated that the leaf apoplastic pH was about 5.5 in non-stressed 
      conditions, and water deficit could trigger an increase in pH by about half a pH 
      unit, which is the first evidence to directly indicate that pH is able to act as 
      a systematic signal under water deficit conditions.
FAU - Li, Bing-Bing
AU  - Li BB
AD  - College of Agronomy and Biotechnology, China Agricultural University, Beijing 
      100094, China.
FAU - Gao, Zhi-Hui
AU  - Gao ZH
FAU - Zhou, Xiao-Yan
AU  - Zhou XY
FAU - Ren, Hui-Bo
AU  - Ren HB
FAU - Xie, Min
AU  - Xie M
FAU - Fan, Yi-Juan
AU  - Fan YJ
FAU - Hu, Jian-Fang
AU  - Hu JF
FAU - Jia, Wen-Suo
AU  - Jia WS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China (Republic : 1949- )
TA  - J Integr Plant Biol
JT  - Journal of integrative plant biology
JID - 101250502
RN  - 0 (Buffers)
RN  - 0 (Fluoresceins)
RN  - 0 (Plant Exudates)
RN  - 059QF0KO0R (Water)
RN  - 85138-49-4 (2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein)
SB  - IM
MH  - Buffers
MH  - Calibration
MH  - Commelina/cytology/metabolism
MH  - Fluoresceins/metabolism
MH  - Helianthus/cytology/metabolism
MH  - Hydrogen-Ion Concentration
MH  - Solanum lycopersicum/cytology/metabolism
MH  - Microscopy, Confocal/*methods
MH  - *Plant Cells
MH  - Plant Epidermis/cytology/metabolism
MH  - Plant Exudates/metabolism
MH  - Plant Roots/metabolism
MH  - Plant Shoots/metabolism
MH  - Plants/*metabolism
MH  - Regression Analysis
MH  - *Signal Transduction
MH  - Time Factors
MH  - Water/metabolism
EDAT- 2008/08/21 09:00
MHDA- 2008/11/04 09:00
CRDT- 2008/08/21 09:00
PHST- 2008/08/21 09:00 [pubmed]
PHST- 2008/11/04 09:00 [medline]
PHST- 2008/08/21 09:00 [entrez]
AID - JIPB667 [pii]
AID - 10.1111/j.1744-7909.2008.00667.x [doi]
PST - ppublish
SO  - J Integr Plant Biol. 2008 Jun;50(6):682-90. doi: 
      10.1111/j.1744-7909.2008.00667.x.