PMID- 18714294
OWN - NLM
STAT- MEDLINE
DCOM- 20081208
LR  - 20080820
IS  - 1750-2799 (Electronic)
IS  - 1750-2799 (Linking)
VI  - 3
IP  - 8
DP  - 2008
TI  - Cultivating previously uncultured soil bacteria using a soil substrate membrane 
      system.
PG  - 1261-9
LID - 10.1038/nprot.2008.102 [doi]
AB  - Most bacteria are recalcitrant to traditional cultivation in the laboratory. The 
      soil substrate membrane system provides a simulated environment for the 
      cultivation of previously undescribed soil bacteria as microcolonies. The system 
      uses a polycarbonate membrane as a solid support for growth and soil extract as 
      the substrate. Diverse microcolonies can be visualized using total bacterial 
      staining combined with fluorescence in situ hybridization (FISH) after 7-10-d 
      incubation. Molecular typing shows that the majority of microcolony-forming 
      bacteria recovered using this protocol were resistant to growth using standard 
      methods. The protocol takes <4 h of bench time over the 10-d period.
FAU - Ferrari, Belinda C
AU  - Ferrari BC
AD  - School of Biotechnology and Biomolecular Sciences, University of New South Wales, 
      Sydney, New South Wales 2052, Australia. b.ferrari@unsw.edu.au
FAU - Winsley, Tristrom
AU  - Winsley T
FAU - Gillings, Michael
AU  - Gillings M
FAU - Binnerup, Svend
AU  - Binnerup S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nat Protoc
JT  - Nature protocols
JID - 101284307
RN  - 0 (Culture Media)
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Bacteria/genetics/growth & development/isolation & purification
MH  - Bacterial Typing Techniques
MH  - *Bacteriological Techniques/instrumentation
MH  - Culture Media
MH  - DNA, Bacterial/chemistry
MH  - In Situ Hybridization, Fluorescence
MH  - *Soil Microbiology
EDAT- 2008/08/21 09:00
MHDA- 2008/12/17 09:00
CRDT- 2008/08/21 09:00
PHST- 2008/08/21 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/08/21 09:00 [entrez]
AID - nprot.2008.102 [pii]
AID - 10.1038/nprot.2008.102 [doi]
PST - ppublish
SO  - Nat Protoc. 2008;3(8):1261-9. doi: 10.1038/nprot.2008.102.