PMID- 18714676
OWN - NLM
STAT- MEDLINE
DCOM- 20090122
LR  - 20190608
IS  - 0963-6897 (Print)
IS  - 0963-6897 (Linking)
VI  - 17
IP  - 5
DP  - 2008
TI  - Improved glucose regulation on a low carbohydrate diet in diabetic rats 
      transplanted with macroencapsulated porcine islets.
PG  - 567-75
AB  - Islet xenografts from porcine donors can reverse diabetes in experimental animal 
      models and may be an alternative to human islet transplantation. We have recently 
      reported the ability of porcine islets encapsulated in a double layer of 
      hydrophilic agarose to maintain in vitro functional ability for >6 months. 
      Although beta-cells are capable of adapting their secretory capacity in response 
      to glucose levels, evidence has shown that prolonged hyperglycemia can compromise 
      this ability. The aim of the present study was to determine the effects of diet 
      manipulation on the long-term regulation of blood glucose levels, and the 
      preservation of functional islet in the macrobeads. Twenty-one 
      streptozotocin-induced diabetic Wistar-Furth male rats were randomly assigned to 
      two diets containing 64% carbohydrate (CHO) or 20% CHO. Groups of five to six 
      animals assigned to either diet were implanted with either empty (EM) or porcine 
      islet-containing macrobeads (PIM) and followed for 333 days. Observations 
      included general condition, body weight, blood glucose, and food and water 
      intakes. Monthly blood samples were collected for insulin and C-peptide analysis. 
      The 20% CHO diet significantly lowered blood glucose values when compared with 
      those of the 64% CHO groups for both the empty (14.94 +/- 0.41 vs. 16.26 +/- 0.42 
      mmol/L, respectively, p < 0.001) and islet macrobead recipients (12.88 +/- 0.39 
      vs. 15.57 +/-0.85 mmol/L, respectively, p <0.001). The different diets, however, 
      had no statistically significant effects on the preservation of islet mass in the 
      macrobead. Serum porcine C-peptide was detected throughout the experiment in 
      animals receiving porcine islet macrobeads, regardless of diet. Diabetic rats fed 
      a low carbohydrate level diet and transplanted with porcine islet macrobeads had 
      improved total tissue glucose disposal and improved clinical parameters 
      associated with diabetes.
FAU - Vinerean, Horatiu V
AU  - Vinerean HV
AD  - Rogosin Institute, Xenia Division, Xenia, OH 45385, USA. hvinerean@rixd.org
FAU - Gazda, Lawrence S
AU  - Gazda LS
FAU - Hall, Richard D
AU  - Hall RD
FAU - Rubin, Albert L
AU  - Rubin AL
FAU - Smith, Barry H
AU  - Smith BH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell Transplant
JT  - Cell transplantation
JID - 9208854
RN  - 0 (Blood Glucose)
RN  - 0 (Capsules)
RN  - 0 (Insulin)
RN  - 9012-36-6 (Sepharose)
SB  - IM
MH  - Animals
MH  - Blood Glucose/*metabolism
MH  - Capsules
MH  - Diabetes Mellitus/diet therapy/metabolism/surgery
MH  - Diabetes Mellitus, Experimental/*diet therapy/metabolism/*surgery
MH  - *Diet, Carbohydrate-Restricted
MH  - Glucose Tolerance Test
MH  - Humans
MH  - Insulin/blood
MH  - *Islets of Langerhans Transplantation
MH  - Male
MH  - Rats
MH  - Rats, Inbred BB
MH  - Rats, Inbred WF
MH  - Sepharose
MH  - Swine
MH  - Transplantation, Heterologous
EDAT- 2008/08/22 09:00
MHDA- 2009/01/23 09:00
CRDT- 2008/08/22 09:00
PHST- 2008/08/22 09:00 [pubmed]
PHST- 2009/01/23 09:00 [medline]
PHST- 2008/08/22 09:00 [entrez]
AID - 10.3727/096368908785095962 [doi]
PST - ppublish
SO  - Cell Transplant. 2008;17(5):567-75. doi: 10.3727/096368908785095962.