PMID- 18714681
OWN - NLM
STAT- MEDLINE
DCOM- 20081219
LR  - 20091119
IS  - 0177-5103 (Print)
IS  - 0177-5103 (Linking)
VI  - 80
IP  - 1
DP  - 2008 Jun 19
TI  - First report of a Mikrocytos-like parasite in European oysters Ostrea edulis from 
      Canada after transport and quarantine in France.
PG  - 27-35
LID - 10.3354/dao01922 [doi]
AB  - As part of a disease resistance experiment, 112 apparently healthy European flat 
      oysters Ostrea edulis L. were exported from Canada (Nova Scotia) into France to 
      test their susceptibility to Bonamia ostreae infection. Twelve oysters died in 
      transit and 17 others died within 2 wk of laboratory quarantine acclimation. All 
      oysters were examined histologically, and the 17 that died during quarantine were 
      assayed for microcells (Bonamia sp. and Mikrocytos mackini) using molecular 
      techniques. A microcell parasite was detected in the connective tissue of 5 of 
      the 112 oysters. Morphological appearance, tissue affinity and molecular 
      characterization through PCR, in situ hybridization (ISH), fluorescence in situ 
      hybridization (FISH) and sequencing revealed a protist related to M. mackini. 
      This is the first report of a parasite of the genus Mikrocytos in a species 
      belonging to the genus Ostrea from the Atlantic Ocean.
FAU - Gagne, N
AU  - Gagne N
AD  - Fisheries and Oceans Canada, Gulf Fisheries Center, PO Box 5030, Moncton, New 
      Brunswick E1C 9B6, Canada. gagnena@dfo-mpo.gc.ca
FAU - Cochennec, N
AU  - Cochennec N
FAU - Stephenson, M
AU  - Stephenson M
FAU - McGladdery, S
AU  - McGladdery S
FAU - Meyer, G R
AU  - Meyer GR
FAU - Bower, S M
AU  - Bower SM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Dis Aquat Organ
JT  - Diseases of aquatic organisms
JID - 8807037
SB  - IM
MH  - Animals
MH  - Atlantic Ocean
MH  - Base Sequence
MH  - Crassostrea/parasitology
MH  - Eukaryota/*isolation & purification/*physiology
MH  - France
MH  - Heart/parasitology
MH  - In Situ Hybridization
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Nova Scotia
MH  - Ostrea/*parasitology
MH  - Polymerase Chain Reaction
MH  - *Quarantine
MH  - Sequence Alignment
MH  - *Transportation
EDAT- 2008/08/22 09:00
MHDA- 2008/12/20 09:00
CRDT- 2008/08/22 09:00
PHST- 2008/08/22 09:00 [pubmed]
PHST- 2008/12/20 09:00 [medline]
PHST- 2008/08/22 09:00 [entrez]
AID - 10.3354/dao01922 [doi]
PST - ppublish
SO  - Dis Aquat Organ. 2008 Jun 19;80(1):27-35. doi: 10.3354/dao01922.