PMID- 18716851
OWN - NLM
STAT- MEDLINE
DCOM- 20090518
LR  - 20211020
IS  - 1543-706X (Electronic)
IS  - 1071-2690 (Linking)
VI  - 44
IP  - 10
DP  - 2008 Nov-Dec
TI  - The frequency, growth kinetics, and osteogenic/adipogenic differentiation 
      properties of canine bone marrow stromal cells.
PG  - 472-9
LID - 10.1007/s11626-008-9137-6 [doi]
AB  - Bone marrow stromal cells (BMSCs) have gained considerable attention as a 
      potential source for cell transplantation therapies for a variety of diseases due 
      to their accessibility, proliferative capacity, and multilineage differentiation 
      properties. Canine BMSCs have been shown to contribute to regeneration of osseous 
      tissues, but knowledge about their biology is currently limited. In the present 
      study, we investigated the frequency of adult canine BMSCs in bone marrow, 
      morphological features, growth kinetics, and osteogenic as well as adipogenic 
      differentiation properties in vitro. Our data suggest that adult canine bone 
      marrow contains approximately one BMSC in every 2.38 x 10(4) bone marrow 
      mononucleated cells (0.0042 +/- 0.0019%, n = 5). Primary BMSC cultures consisted 
      of morphologically heterogeneous adherent cell populations from which 
      spindle-shaped cells grew and became the predominant cell type. Growth kinetics 
      patterns were dependent on the initial cell seeding densities, resulting in the 
      highest fold increase at lower cell density. In the presence of osteogenic and 
      adipogenic inducers, primary BMSCs underwent morphological and phenotypic changes 
      characteristic of osteogenic and adipogenic differentiation, respectively. This 
      study provides insights into basic characterization of adult canine BMSCs.
FAU - Kamishina, Hiroaki
AU  - Kamishina H
AD  - Department of Small Animal Clinical Sciences, College of Veterinary Medicine, 
      University of Florida, Gainesville, FL 32610-0126, USA. Kamicna@iwate-u.ac.jp
FAU - Farese, James P
AU  - Farese JP
FAU - Storm, Joshua A
AU  - Storm JA
FAU - Cheeseman, Jennifer A
AU  - Cheeseman JA
FAU - Clemmons, Roger M
AU  - Clemmons RM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080821
PL  - Germany
TA  - In Vitro Cell Dev Biol Anim
JT  - In vitro cellular & developmental biology. Animal
JID - 9418515
SB  - IM
MH  - *Adipogenesis
MH  - Animals
MH  - Bone Marrow Cells/*cytology
MH  - Cell Proliferation
MH  - Cell Shape
MH  - Colony-Forming Units Assay
MH  - Dogs
MH  - Female
MH  - Kinetics
MH  - Male
MH  - *Osteogenesis
MH  - Stromal Cells/*cytology
EDAT- 2008/08/22 09:00
MHDA- 2009/05/19 09:00
CRDT- 2008/08/22 09:00
PHST- 2008/02/15 00:00 [received]
PHST- 2008/07/16 00:00 [accepted]
PHST- 2008/08/22 09:00 [pubmed]
PHST- 2009/05/19 09:00 [medline]
PHST- 2008/08/22 09:00 [entrez]
AID - 10.1007/s11626-008-9137-6 [doi]
PST - ppublish
SO  - In Vitro Cell Dev Biol Anim. 2008 Nov-Dec;44(10):472-9. doi: 
      10.1007/s11626-008-9137-6. Epub 2008 Aug 21.