PMID- 18717958
OWN - NLM
STAT- MEDLINE
DCOM- 20080930
LR  - 20121115
IS  - 1600-0781 (Electronic)
IS  - 0905-4383 (Linking)
VI  - 24
IP  - 4
DP  - 2008 Aug
TI  - Protective effect of the Baicalin against DNA damage induced by ultraviolet B 
      irradiation to mouse epidermis.
PG  - 175-82
LID - 10.1111/j.1600-0781.2008.00356.x [doi]
AB  - PURPOSE: To investigate whether topical application of Baicalin affords 
      protecting Balb/C mice epidermis from ultraviolet (UV)B-induced DNA damage and 
      its underlying mechanisms. METHODS: A DNA damage model of UVB irradiation-induced 
      mice epidermis was established. The immunohistochemical staining, Southwestern 
      dot-blotting were used for cyclobutane pyrimidine dimers (CPDs) detection; 
      Western blotting was used for p53 detection; reverse transcription-polymerase 
      chain reaction (RT-PCR) was used to detect the mRNA level of Bcl-2 and Bax; 
      terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) was 
      used to detect apoptotic cells. RESULTS: Topical application of Baicalin on 
      Balb/C mice skin significantly decreased the amount of epidermal CPDs 1, 24 and 
      48 h after 180 mJ/cm(2) of UVB irradiation as compared with untreated mice. 
      UVB-induced apoptosis was less pronounced in Baicalin-treated mice epidermis, 
      which was accompanied by less p53 accumulation and higher Bcl-2/Bax ratio 
      compared with that of untreated mice. CONCLUSION: Taken together, these results 
      suggest that topical Baicalin application mitigates DNA photo-damage. Baicalin is 
      therefore a promising protective substance against UVB radiation.
FAU - Bing-Rong, Zhou
AU  - Bing-Rong Z
AD  - Department of Dermatology, First Affiliated Hospital of Nanjing Medical 
      University, Nanjing, China.
FAU - Song-Liang, Jin
AU  - Song-Liang J
FAU - Xiao-E, Chen
AU  - Xiao-E C
FAU - Xiang-Fei, Lin
AU  - Xiang-Fei L
FAU - Bao-Xiang, Cai
AU  - Bao-Xiang C
FAU - Jie, Gao
AU  - Jie G
FAU - Dan, Luo
AU  - Dan L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Photodermatol Photoimmunol Photomed
JT  - Photodermatology, photoimmunology & photomedicine
JID - 9013641
RN  - 0 (Bax protein, mouse)
RN  - 0 (Flavonoids)
RN  - 0 (Plant Extracts)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Pyrimidine Dimers)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 347Q89U4M5 (baicalin)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Administration, Cutaneous
MH  - Analysis of Variance
MH  - Animals
MH  - Apoptosis/drug effects/radiation effects
MH  - DNA/analysis
MH  - DNA Damage/*drug effects
MH  - Epidermis/*drug effects/metabolism/physiology/*radiation effects
MH  - Female
MH  - Flavonoids/*pharmacology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Plant Extracts/*pharmacology
MH  - Proto-Oncogene Proteins c-bcl-2/genetics/metabolism
MH  - Pyrimidine Dimers/metabolism
MH  - RNA, Messenger/analysis
MH  - *Scutellaria baicalensis
MH  - Tumor Suppressor Protein p53/metabolism
MH  - Ultraviolet Rays/*adverse effects
MH  - bcl-2-Associated X Protein/genetics/metabolism
EDAT- 2008/08/23 09:00
MHDA- 2008/10/01 09:00
CRDT- 2008/08/23 09:00
PHST- 2008/08/23 09:00 [pubmed]
PHST- 2008/10/01 09:00 [medline]
PHST- 2008/08/23 09:00 [entrez]
AID - PPP356 [pii]
AID - 10.1111/j.1600-0781.2008.00356.x [doi]
PST - ppublish
SO  - Photodermatol Photoimmunol Photomed. 2008 Aug;24(4):175-82. doi: 
      10.1111/j.1600-0781.2008.00356.x.