PMID- 18717968
OWN - NLM
STAT- MEDLINE
DCOM- 20080925
LR  - 20081121
IS  - 0931-2668 (Print)
IS  - 0931-2668 (Linking)
VI  - 125
IP  - 4
DP  - 2008 Aug
TI  - Bovine SLC11A1 3' UTR SSCP genotype evaluated by a macrophage in vitro killing 
      assay employing a Brucella abortus strain.
PG  - 271-9
LID - 10.1111/j.1439-0388.2008.00727.x [doi]
AB  - The 3' untranslated region (3' UTR) of the bovine natural resistance-associated 
      macrophage gene (NRAMP1 or SLC11A1) was genotyped in Colombian Creole Blanco 
      Orejinegro (BON) (Bos taurus) (n = 140) and Zebu Brahman (Bos indicus) (Z) (n = 
      20) cattle and their crosses (BON x Zebu Brahman [B x Z] [n = 10]; Zebu Brahman x 
      BON [Z x B] [n = 10]), and in animals from a Holstein x BON (H x B) (n = 10) 
      cross. Direct sequencing and single-strand conformation polymorphism analysis 
      (SSCP) helped in detecting the polymorphic behaviour. The association between 
      resistance to brucellosis infection and SSCP genotype was evaluated using a 
      macrophage in vitro killing assay employing a virulent Brucella abortus strain. 
      The 3' UTR (GT) repeated polymorphism was gentoyped and its association with 
      resistance to brucellosis was evaluated. When all breeds were grouped, a high 
      frequency in the homozygote GT(12) (AA genotype) (0.823) and a very low frequency 
      in the homozygote GT(10) (BB genotype) (0.047) were detected. The BON (0.963), Z 
      x B (0.60) and H x B (1.00) cattle showed high GT(12) allele frequencies, unlike 
      that seen for the B x Z and Zebu cattle (0.3002 and 0.218, respectively). The 
      GT(10) allele was only found in the Zebu cattle (0.391). A significant 
      association (p < 0.001) was found between the B. abortus macrophage in vitro 
      killing assay phenotypes and the bovine SLC11A1 3' UTR genotypes, which suggests 
      that the A allele may be associated with resistance. Because only nine animals 
      had the BB genotype, the results require some confirmation in more extensive 
      populations.
FAU - Martinez, R
AU  - Martinez R
AD  - Grupo de Recursos Geneticos y Biotecnologia Animal, Centro de Biotecnologia y 
      Bioindustria, Corporacion Colombiana de Investigacion Agropecuaria (CORPOICA), CI 
      Tibaitata Km, Bogota DC, Colombia. rodrigomartinez19@yahoo.com 
      <rodrigomartinez19@yahoo.com>
FAU - Toro, R
AU  - Toro R
FAU - Montoya, F
AU  - Montoya F
FAU - Burbano, M
AU  - Burbano M
FAU - Tobon, J
AU  - Tobon J
FAU - Gallego, J
AU  - Gallego J
FAU - Dunner, S
AU  - Dunner S
FAU - Canon, J
AU  - Canon J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - J Anim Breed Genet
JT  - Journal of animal breeding and genetics = Zeitschrift fur Tierzuchtung und 
      Zuchtungsbiologie
JID - 100955807
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Cation Transport Proteins)
RN  - 0 (Genetic Markers)
RN  - 0 (natural resistance-associated macrophage protein 1)
SB  - IM
MH  - 3' Untranslated Regions/*chemistry
MH  - Animals
MH  - Brucella abortus/*pathogenicity
MH  - Brucellosis, Bovine/genetics/immunology
MH  - Cation Transport Proteins/*genetics
MH  - Cattle/*genetics
MH  - Crosses, Genetic
MH  - Gene Frequency
MH  - Genetic Markers
MH  - Genotype
MH  - Immunity, Innate/genetics
MH  - Macrophages/physiology
MH  - Microsatellite Repeats
MH  - *Polymorphism, Single-Stranded Conformational
MH  - Sequence Analysis, DNA
EDAT- 2008/08/23 09:00
MHDA- 2008/09/26 09:00
CRDT- 2008/08/23 09:00
PHST- 2008/08/23 09:00 [pubmed]
PHST- 2008/09/26 09:00 [medline]
PHST- 2008/08/23 09:00 [entrez]
AID - JBG727 [pii]
AID - 10.1111/j.1439-0388.2008.00727.x [doi]
PST - ppublish
SO  - J Anim Breed Genet. 2008 Aug;125(4):271-9. doi: 10.1111/j.1439-0388.2008.00727.x.