PMID- 18718171
OWN - NLM
STAT- MEDLINE
DCOM- 20090515
LR  - 20080822
IS  - 1745-7254 (Electronic)
IS  - 1671-4083 (Linking)
VI  - 29
IP  - 9
DP  - 2008 Sep
TI  - Differentiations of transplanted mouse spermatogonial stem cells in the adult 
      mouse renal parenchyma in vivo.
PG  - 1029-34
LID - 10.1111/j.1745-7254.2008.00836.x [doi]
AB  - AIM: Spermatogonial stem cells can initiate the process of cellular 
      differentiation to generate mature spermatozoa, but whether it possess the 
      characteristic of pluripotency and plasticity, similar to embryonic stem cells, 
      has not been elucidated. This study was designed to evaluate the differentiation 
      potential of spermatogonial stem cells into renal cells in vivo. METHODS: 
      Neonatal mouse spermatogonial stem cells were transplanted into mature male mice 
      lacking endogenous spermatogenesis. The restoration of fertility in recipient 
      males was observed. Spermatogonial stem cells were then injected into renal 
      parenchyma of mature female mice to make a new extracellular environment for 
      differentiation. Fluorescence in situ hybridization technology (FISH) was used to 
      detect the expression of chromosome Y in recipient renal tissues. To determine 
      the type of cells differentiated from spermatogonial stem cells, the expression 
      of ricinus communis agglutinin, vimentin, CD45, and F(4/80) proteins were 
      examined in the renal tissues by immunohistochemistry. RESULTS: The proliferation 
      of seminiferous epithelial cells was distinctly observed in seminiferous tubules 
      of transplanted testes, whereas no regeneration of spermatogenesis was observed 
      in non-transplanted control testes. In transplanted female renal tissues, FISH 
      showed a much stronger immuno-fluorescence signal of chromosome Y in the 
      nucleolus of epithelial cells of the renal tubule and podocytes of the 
      glomerulus. CONCLUSION: The spermatogonial stem cells were successfully purified 
      from mouse testicles. This finding demonstrated that spermatogonial stem cells 
      could not only restore damaged spermatogenesis, but were also capable of 
      differentiating into mature renal parenchyma cells in vivo.
FAU - Wu, Da-peng
AU  - Wu DP
AD  - Department of Urology, First Affiliated Hospital, Medical School of Xi-an 
      Jiaotong University, Xi-an 710061, China.
FAU - He, Da-lin
AU  - He DL
FAU - Li, Xiang
AU  - Li X
FAU - Liu, Zhao-hui
AU  - Liu ZH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Acta Pharmacol Sin
JT  - Acta pharmacologica Sinica
JID - 100956087
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Cell Differentiation/*physiology
MH  - Gene Expression Regulation
MH  - Kidney/*cytology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Pluripotent Stem Cells/physiology
MH  - Spermatogonia/*physiology/*transplantation
MH  - *Stem Cell Transplantation
MH  - Testis/cytology
MH  - X Chromosome/genetics
EDAT- 2008/08/23 09:00
MHDA- 2009/05/16 09:00
CRDT- 2008/08/23 09:00
PHST- 2008/08/23 09:00 [pubmed]
PHST- 2009/05/16 09:00 [medline]
PHST- 2008/08/23 09:00 [entrez]
AID - 10.1111/j.1745-7254.2008.00836.x [doi]
PST - ppublish
SO  - Acta Pharmacol Sin. 2008 Sep;29(9):1029-34. doi: 
      10.1111/j.1745-7254.2008.00836.x.