PMID- 18721270
OWN - NLM
STAT- MEDLINE
DCOM- 20081020
LR  - 20080825
IS  - 0001-2815 (Print)
IS  - 0001-2815 (Linking)
VI  - 72
IP  - 2
DP  - 2008 Aug
TI  - Loss of heterozygosity at 6p21 underlying [corrected] HLA class I downregulation 
      in Chinese primary esophageal squamous cell carcinomas.
PG  - 105-14
LID - 10.1111/j.1399-0039.2008.01078.x [doi]
AB  - Loss or downregulation of human leukocyte antigen (HLA) class I molecules is a 
      widespread mechanism used by tumor cells to avoid tumor recognition by cytotoxic 
      T lymphocytes favoring tumor immune escape. Multiple molecular mechanisms are 
      responsible for these altered HLA class I tumor phenotypes, such as the loss of 
      heterozygosity (LOH) at chromosome region 6p21.3. In this study, we used 
      immunohistological techniques with a highly selective panel of anti-HLA 
      monoclonal antibodies to analyze the expression of HLA class I molecules in 84 
      formalin-fixed, paraffin-embedded section and 49 frozen-fresh tissues of primary 
      esophageal squamous cell carcinomas (pESCC) from Chinese patients. To elucidate 
      the underlying mechanism of HLA class I loss or downregulation, we also analyzed 
      LOH of previously selected microsatellite markers located in chromosomes 6 and 15 
      by polymerase chain reaction. DNA was obtained from frozen-fresh tumor tissues 
      and surrounding stroma to define the LOH associated with chromosomes 6p21 and 
      15q21. Our results showed that HLA-A, HLA-B/C, HLA class I heavy chain, 
      beta2-microglobuline, and HLA class I complex were lost or downregulated in pESCC 
      (P<0.0001), and were moderately associated with the microsatellite alterations in 
      HLA class I gene regions, correlated with patients' age, tumor's location, and 
      stage, and indicated that LOH at 6p21.3 is a frequent mechanism that leads to HLA 
      class I abnormalities in pESCC.
FAU - Yang, Y
AU  - Yang Y
AD  - Key Laboratory of Developmental Genes and Human Disease of Education Ministry, 
      Department of Genetics and Developmental Biology, Southeast University Medical 
      School, Nanjing, Jiangsu, China.
FAU - Zhang, J
AU  - Zhang J
FAU - Miao, F
AU  - Miao F
FAU - Wei, J
AU  - Wei J
FAU - Shen, C
AU  - Shen C
FAU - Shen, Y
AU  - Shen Y
FAU - Xie, W
AU  - Xie W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma, Squamous Cell/*genetics/immunology/pathology
MH  - Case-Control Studies
MH  - China
MH  - Chromosomes, Human, Pair 15
MH  - *Chromosomes, Human, Pair 6
MH  - Down-Regulation/genetics/immunology
MH  - Esophageal Neoplasms/*genetics/immunology/pathology
MH  - *Genes, MHC Class I
MH  - Histocompatibility Antigens Class I/immunology/metabolism
MH  - Humans
MH  - *Loss of Heterozygosity/immunology
MH  - Microsatellite Repeats
MH  - Middle Aged
MH  - Paraffin Embedding
EDAT- 2008/08/30 09:00
MHDA- 2008/10/22 09:00
CRDT- 2008/08/30 09:00
PHST- 2008/08/30 09:00 [pubmed]
PHST- 2008/10/22 09:00 [medline]
PHST- 2008/08/30 09:00 [entrez]
AID - TAN1078 [pii]
AID - 10.1111/j.1399-0039.2008.01078.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2008 Aug;72(2):105-14. doi: 10.1111/j.1399-0039.2008.01078.x.