PMID- 18722347
OWN - NLM
STAT- MEDLINE
DCOM- 20081027
LR  - 20091119
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 375
IP  - 4
DP  - 2008 Oct 31
TI  - Caveolae as potential mediators of MCH-signaling pathways.
PG  - 592-5
LID - 10.1016/j.bbrc.2008.08.038 [doi]
AB  - The melanin-concentrating hormone receptor (MCHR) 1 is a G protein-coupled 
      receptor involved in the regulation of appetite and energy expenditure in 
      mammals. Here, we show that MCHR1 partitions to lipid rafts in stably expressing 
      Chinese hamster ovary cells. In addition to co-fractionating with lipid rafts 
      containing caveolin-1 on sucrose gradients, caveolin-1 was present in MCHR1 
      immunoprecipitates, suggesting that MCHR1 complexes with caveolae. The 
      cholesterol-depleting drug methyl-beta-cyclodextrin impaired MCH-mediated ERK 
      signaling. These data suggest that a functional interaction between MCHR1 and 
      caveolin-1 in lipid rafts exists and provide a basis for further biochemical 
      studies to understand the significance on MCH-mediated signal transduction 
      events.
FAU - Cook, Laurie B
AU  - Cook LB
AD  - Department of Biological Sciences, 217 Lennon Hall, The College at Brockport, 
      State University of New York, 350 New Campus Drive, Brockport, NY 14420, USA. 
      lcook@brockport.edu
FAU - Delorme-Axford, Elizabeth B
AU  - Delorme-Axford EB
FAU - Robinson, Kelsi
AU  - Robinson K
LA  - eng
PT  - Journal Article
DEP - 20080821
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Caveolin 1)
RN  - 0 (MCHR1 protein, human)
RN  - 0 (Receptors, Somatostatin)
RN  - 0 (beta-Cyclodextrins)
RN  - 0 (methyl-beta-cyclodextrin)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
SB  - IM
MH  - Animals
MH  - CHO Cells
MH  - Caveolae/drug effects/*metabolism
MH  - Caveolin 1/*metabolism
MH  - Cricetinae
MH  - Cricetulus
MH  - Humans
MH  - Immunoprecipitation
MH  - Membrane Microdomains/*metabolism
MH  - Mitogen-Activated Protein Kinase 1/metabolism
MH  - Mitogen-Activated Protein Kinase 3/metabolism
MH  - Receptors, Somatostatin/genetics/*metabolism
MH  - Signal Transduction
MH  - beta-Cyclodextrins/pharmacology
EDAT- 2008/08/30 09:00
MHDA- 2008/10/28 09:00
CRDT- 2008/08/30 09:00
PHST- 2008/08/11 00:00 [received]
PHST- 2008/08/12 00:00 [accepted]
PHST- 2008/08/30 09:00 [pubmed]
PHST- 2008/10/28 09:00 [medline]
PHST- 2008/08/30 09:00 [entrez]
AID - S0006-291X(08)01589-1 [pii]
AID - 10.1016/j.bbrc.2008.08.038 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2008 Oct 31;375(4):592-5. doi: 
      10.1016/j.bbrc.2008.08.038. Epub 2008 Aug 21.