PMID- 18723417
OWN - NLM
STAT- MEDLINE
DCOM- 20090330
LR  - 20181201
IS  - 1878-7568 (Electronic)
IS  - 1742-7061 (Linking)
VI  - 5
IP  - 1
DP  - 2009 Jan
TI  - Gelatin/chitosan/hyaluronan scaffold integrated with PLGA microspheres for 
      cartilage tissue engineering.
PG  - 328-37
LID - 10.1016/j.actbio.2008.07.030 [doi]
AB  - Poly(lactide-co-glycotide) (PLGA) microspheres integrated into 
      gelatin/chitosan/hyaluronan scaffolds were fabricated by freeze-drying and 
      crosslinking with 1-ethyl-3-(3-dimethyl aminopropyl)carbodiimide. The effects of 
      the microspheres on porosity, density, compressive modulus, phosphate-buffered 
      saline uptake ratio and weight loss of the scaffolds were evaluated. Generally, a 
      scaffold with a higher PLGA content had a lower porosity and weight loss, and a 
      medium uptake ratio, but a larger apparent density and compressive modulus. When 
      the PLGA content was lower than 50%, the PLGA-integrated scaffolds had a similar 
      pore size (approximately 200microm) as that of the control, and as much as 90% of 
      their porosity could be preserved. In vitro chondrocyte culture in the 50% 
      PLGA-integrated scaffold demonstrated that the cells could proliferate and 
      secrete extracellular matrix at the same level as in the control 
      gelatin/chitosan/hyaluronan scaffold.
FAU - Tan, Huaping
AU  - Tan H
AD  - Key Laboratory of Macromolecular Synthesis and Functionalization, Ministry of 
      Education, and Department of Polymer Science and Engineering, Zhejiang 
      University, Hangzhou 310027, China.
FAU - Wu, Jindan
AU  - Wu J
FAU - Lao, Lihong
AU  - Lao L
FAU - Gao, Changyou
AU  - Gao C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080806
PL  - England
TA  - Acta Biomater
JT  - Acta biomaterialia
JID - 101233144
RN  - 0 (Biocompatible Materials)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 9000-70-8 (Gelatin)
RN  - 9004-61-9 (Hyaluronic Acid)
RN  - 9012-76-4 (Chitosan)
SB  - IM
MH  - Animals
MH  - Biocompatible Materials/*chemistry
MH  - Cartilage/*metabolism
MH  - Cell Proliferation
MH  - Chitosan/*administration & dosage
MH  - Chondrocytes/metabolism
MH  - Compressive Strength
MH  - Gelatin/*administration & dosage
MH  - Hyaluronic Acid/*administration & dosage
MH  - Lactic Acid/*chemistry
MH  - Microspheres
MH  - Models, Biological
MH  - Polyglycolic Acid/*chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Porosity
MH  - Rabbits
MH  - Tissue Engineering/*methods
EDAT- 2008/08/30 09:00
MHDA- 2009/03/31 09:00
CRDT- 2008/08/30 09:00
PHST- 2008/01/16 00:00 [received]
PHST- 2008/07/09 00:00 [revised]
PHST- 2008/07/15 00:00 [accepted]
PHST- 2008/08/30 09:00 [pubmed]
PHST- 2009/03/31 09:00 [medline]
PHST- 2008/08/30 09:00 [entrez]
AID - S1742-7061(08)00217-1 [pii]
AID - 10.1016/j.actbio.2008.07.030 [doi]
PST - ppublish
SO  - Acta Biomater. 2009 Jan;5(1):328-37. doi: 10.1016/j.actbio.2008.07.030. Epub 2008 
      Aug 6.