PMID- 18724668
OWN - NLM
STAT- MEDLINE
DCOM- 20100617
LR  - 20220331
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 26
IP  - 3
DP  - 2008 May
TI  - [Determination of ifenprodil in human plasma using liquid chromatography with 
      electrospray tandem mass spectrometry].
PG  - 322-6
AB  - A method for the determination of ifenprodil levels in human plasma was 
      established. Ifenprodil and the internal standard (IS), ketoconazole, were 
      extracted from the plasma with ethyl acetate using liquid-liquid extraction. The 
      extracts were separated by high performance liquid chromatography (HPLC) using 
      methanol-6 mmol/L ammonium acetate (pH value was adjusted to 7.40) (90 : 10, v/v) 
      as the mobile phase, and were then detected using mass spectrometry (MS). 
      Electrospray source was applied and operated in positive ion mode. Selected 
      reaction monitoring (SRM) mode with the transition of m/z 326.1 --> 308.2 was 
      used to quantify ifenprodil, and m/z 531.0 --> 82.1 for IS. The excellent 
      sensitivity and selectivity of the HPLC-MS/MS method allowed quantitation and 
      identification of ifenprodil at low levels with a run time of 6.0 min. The assay 
      was linear over the range from 0.25 to 50 microg/L. The intra-day and inter-day 
      precisions measured as relative standard deviations (RSDs) were less than 2.7% 
      and less than 6.5%, respectively. The average recoveries varied between 101.3% 
      and 105.0%, and the detection limit was 0.08 microg/L. Due to its simplicity and 
      accuracy, the established method is suitable for the application in a 
      pharmaceutical study of the intervenous drop infusion of ifenprodil tartrate.
FAU - Yang, Bo
AU  - Yang B
AD  - Department of Pharmacy, Southwest Hospital, Third Military Medical University, 
      Chongqing 400038, China. tmmuyb1@163.com
FAU - Chen, Yongchuan
AU  - Chen Y
FAU - Xiang, Rongfeng
AU  - Xiang R
FAU - Yang, Dan
AU  - Yang D
FAU - Xia, Peiyuan
AU  - Xia P
FAU - Liu, Songqing
AU  - Liu S
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Piperidines)
RN  - R8OE3P6O5S (ifenprodil)
SB  - IM
MH  - Blood Chemical Analysis/*methods
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Humans
MH  - Limit of Detection
MH  - Piperidines/*blood/isolation & purification
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
MH  - Tandem Mass Spectrometry/*methods
MH  - Temperature
EDAT- 2008/08/30 09:00
MHDA- 2010/06/18 06:00
CRDT- 2008/08/30 09:00
PHST- 2008/08/30 09:00 [pubmed]
PHST- 2010/06/18 06:00 [medline]
PHST- 2008/08/30 09:00 [entrez]
AID - 10.1016/s1872-2059(08)60019-x [doi]
PST - ppublish
SO  - Se Pu. 2008 May;26(3):322-6. doi: 10.1016/s1872-2059(08)60019-x.