PMID- 18724708
OWN - NLM
STAT- MEDLINE
DCOM- 20090908
LR  - 20191210
IS  - 1000-3061 (Print)
IS  - 1000-3061 (Linking)
VI  - 24
IP  - 5
DP  - 2008 May
TI  - [The rescue of H1N1 subtype swine influenza virus].
PG  - 857-61
AB  - The swine influenza virus (SIV) strain A/Swine/TianJin/01/2004(H1N1) (A/S/TJ/04) 
      was rescued successfully by an eight-plasmid rescue system. The cDNAs of SIV 8 
      gene segments were synthesized by RT-PCR and cloned into the RNA polymerase I/II 
      bidirection expression vector PHW2000 independently, resulting in 8 recombinant 
      plasmids. The 8 recombinant plasmids were cotransfected into COS-1 cell, 30 h 
      later TPCK-trypsin was added to 0.5 microg/mL. The COS-1 cell and supernatant 
      were harvested 48 h after cotransfection and were inoculated into the allantoic 
      cavity of 9-day-old specific-pathogen free (SPF) chicken eggs. The allantoic 
      fluid of dead eggs was harvested and passaged 3 generations in SPF chicken eggs 
      to get infective virus. The successful rescue of A/S/TJ/04 SIV was identified by 
      hemagglutination assay, hemagglutination inhibition assay, sequence analysis and 
      electron microscope observation. The successful rescue of SIV built a platform 
      for the research of the relationship between genome structure and function of 
      SIV, the mechanisms of trans-species transmission of influenza virus and for the 
      generation of new SIV as vaccine.
FAU - Peng, Yaping
AU  - Peng Y
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan 430070, China.
FAU - Zhou, Hongbo
AU  - Zhou H
FAU - Li, Chun
AU  - Li C
FAU - Jin, Meilin
AU  - Jin M
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sheng Wu Gong Cheng Xue Bao
JT  - Sheng wu gong cheng xue bao = Chinese journal of biotechnology
JID - 9426463
RN  - EC 2.7.7.- (RNA Polymerase II)
RN  - EC 2.7.7.6 (RNA Polymerase I)
SB  - IM
MH  - Animals
MH  - COS Cells
MH  - Chick Embryo
MH  - Chickens
MH  - Chlorocebus aethiops
MH  - Influenza A Virus, H1N1 Subtype/*genetics/*physiology
MH  - Plasmids/genetics
MH  - RNA Polymerase I/genetics
MH  - RNA Polymerase II/genetics
MH  - Recombination, Genetic/*genetics
MH  - Swine
MH  - Transfection
MH  - *Virus Replication
EDAT- 2008/08/30 09:00
MHDA- 2009/09/09 06:00
CRDT- 2008/08/30 09:00
PHST- 2008/08/30 09:00 [pubmed]
PHST- 2009/09/09 06:00 [medline]
PHST- 2008/08/30 09:00 [entrez]
PST - ppublish
SO  - Sheng Wu Gong Cheng Xue Bao. 2008 May;24(5):857-61.