PMID- 18725324
OWN - NLM
STAT- MEDLINE
DCOM- 20081203
LR  - 20161124
IS  - 1567-5769 (Print)
IS  - 1567-5769 (Linking)
VI  - 8
IP  - 12
DP  - 2008 Dec 10
TI  - Wogonin inhibits microglial cell migration via suppression of nuclear 
      factor-kappa B activity.
PG  - 1658-62
LID - 10.1016/j.intimp.2008.07.018 [doi]
AB  - Previously, we and others have demonstrated that wogonin, an active component 
      from the root of Scutellaria baicalensis Georgi, has a neuroprotective effect in 
      cerebral ischemic insult. The neuroprotective effect of wogonin may at least in 
      part be due to its anti-inflammatory properties. Microglial cells, well-known 
      residential macrophages in the central nervous system, migrate to the ischemic 
      lesion and play a pivotal role in the development of chronic inflammation. In the 
      present study, we observed that wogonin potently inhibited microglial migration 
      toward a chemokine, monocyte chemoattractant protein-1 (MCP-1). The 
      anti-migratory effect of wogonin was provoked at nanomolar concentrations, at 
      which wogonin did not significantly inhibit the production of cytokines and 
      chemokines. NF-kappaB has previously shown to regulate microglial cell migration, 
      and activation of cAMP-signaling pathway has also been associated with inhibition 
      of microglial cell motility. In the present study, wogonin at low micromolar 
      concentrations completely suppressed the activity of NF-kappaB in 
      MCP-1-stimulated microglia, and NF-kappaB inhibitors such as N-acetyl cysteine 
      and pyrrolidinedithiocarbamate inhibited the MCP-1-induced migration of 
      microglial cells. However, wogonin did not stimulate the production of cAMP in 
      microglial cells. Our results indicate that the anti-inflammatory activity of 
      wogonin is exerted at least in part by suppressing microglial cell motility via 
      inhibition of NF-kappaB activity.
FAU - Piao, Hua Zi
AU  - Piao HZ
AD  - Department of Pharmacology, College of Medicine, Yanbian University, Yanji, 
      China.
FAU - Choi, In Young
AU  - Choi IY
FAU - Park, Jin-Sun
AU  - Park JS
FAU - Kim, Hee-Sun
AU  - Kim HS
FAU - Cheong, Jae Hoon
AU  - Cheong JH
FAU - Son, Kun Ho
AU  - Son KH
FAU - Jeon, Su Jin
AU  - Jeon SJ
FAU - Ko, Kwang Ho
AU  - Ko KH
FAU - Kim, Won-Ki
AU  - Kim WK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080824
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Flavanones)
RN  - 0 (NF-kappa B)
RN  - E0399OZS9N (Cyclic AMP)
RN  - POK93PO28W (wogonin)
SB  - IM
MH  - Animals
MH  - Cell Movement/drug effects
MH  - Chemokine CCL2/antagonists & inhibitors
MH  - Chemokine CCL5/antagonists & inhibitors
MH  - Cyclic AMP/biosynthesis
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - Flavanones/*pharmacology
MH  - Microglia/*drug effects/physiology
MH  - NF-kappa B/*antagonists & inhibitors
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Scutellaria/*chemistry
EDAT- 2008/08/30 09:00
MHDA- 2008/12/17 09:00
CRDT- 2008/08/30 09:00
PHST- 2008/04/30 00:00 [received]
PHST- 2008/07/29 00:00 [accepted]
PHST- 2008/08/30 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/08/30 09:00 [entrez]
AID - S1567-5769(08)00237-3 [pii]
AID - 10.1016/j.intimp.2008.07.018 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2008 Dec 10;8(12):1658-62. doi: 
      10.1016/j.intimp.2008.07.018. Epub 2008 Aug 24.