PMID- 18725347
OWN - NLM
STAT- MEDLINE
DCOM- 20081204
LR  - 20080826
IS  - 0003-3898 (Print)
IS  - 0003-3898 (Linking)
VI  - 66
IP  - 4
DP  - 2008 Jul-Aug
TI  - [Discrepancy between two methods of D-dimers measurement: one case of human 
      anti-mouse antibody interference].
PG  - 441-6
LID - 10.1684/abc.2008.0247 [doi]
AB  - Quantification of D-dimers is the major biometry step in the diagnostic of an 
      episode of the venous thromboembolic disease. The measurement of D-dimers can be 
      performed with ELISA or immunoturbidimetric methods suited to emergency, using a 
      mouse monoclonal antibody as capture and/or revelation antibody. Therefore, the 
      presence in patient's plasma of human antibody mouse (HAMA) that binds the mouse 
      antiglobulin used in immunoassays can lead to false negative or false positive 
      results. In a young woman presenting repetitive thoracic pain suggestive of a 
      pulmonary embolism, a major discrepancy was found between one result of D-dimers 
      above the cut-off with an immunoturbidimetric method (STA Liatest D-DI; 
      Diagnostica Stago) and one result below the cut-off with a sandwich method (Vidas 
      D-Dimer Exclusion; bioMerieux). HAMA, which is known to be responsible for this 
      type of discrepancy, was detected in the patient serum. The false positive result 
      probably impaired with the management of patient. Taking in charge the patient 
      should take into account the possible presence of this antibody. Interference by 
      heterophilic antibodies is not easily detected by the laboratory. Even if their 
      frequency is low, it remains a difficult problem for the biologist. Suspicion 
      generally arises from inconsistency between the clinical data and immunoassay 
      results. A good communication between physician and biologist should avoid to 
      providing false negative or positive results.
FAU - Rouviere, J A
AU  - Rouviere JA
AD  - Service d'hematologie biologique, Centre hospitalier universitaire de Nancy, 
      Vandoeuvre-les-Nancy.
FAU - Devignes, J
AU  - Devignes J
FAU - de Maistre, E
AU  - de Maistre E
FAU - Kennel, A
AU  - Kennel A
FAU - Chabot, F
AU  - Chabot F
FAU - Lecompte, T
AU  - Lecompte T
LA  - fre
PT  - Case Reports
PT  - English Abstract
PT  - Journal Article
TT  - Incoherence entre deux methodes de dosage des D-dimeres: un cas d'interference 
      d'anticorps humain anti-souris.
PL  - France
TA  - Ann Biol Clin (Paris)
JT  - Annales de biologie clinique
JID - 2984690R
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Fibrin Fibrinogen Degradation Products)
RN  - 0 (fibrin fragment D)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*immunology
MH  - Female
MH  - Fibrin Fibrinogen Degradation Products/*analysis
MH  - Humans
MH  - Immunoassay/methods
MH  - Mice
MH  - Pulmonary Embolism/*blood/*immunology
MH  - Young Adult
EDAT- 2008/08/30 09:00
MHDA- 2008/12/17 09:00
CRDT- 2008/08/30 09:00
PHST- 2007/12/21 00:00 [received]
PHST- 2008/04/16 00:00 [accepted]
PHST- 2008/08/30 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/08/30 09:00 [entrez]
AID - abc.2008.0247 [pii]
AID - 10.1684/abc.2008.0247 [doi]
PST - ppublish
SO  - Ann Biol Clin (Paris). 2008 Jul-Aug;66(4):441-6. doi: 10.1684/abc.2008.0247.