PMID- 18725395
OWN - NLM
STAT- MEDLINE
DCOM- 20090122
LR  - 20161124
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 84
IP  - 6
DP  - 2008 Dec
TI  - Human dendritic cells differentiated in hypoxia down-modulate antigen uptake and 
      change their chemokine expression profile.
PG  - 1472-82
LID - 10.1189/jlb.0208082 [doi]
AB  - Dendritic cells (DCs) are the most potent antigen-presenting cells and fine-tune 
      the immune response. We have investigated hypoxia's effects on the 
      differentiation and maturation of DCs from human monocytes in vitro, and have 
      shown that it affects DC functions. Hypoxic immature DCs (H-iDCs) significantly 
      fail to capture antigens through down-modulation of the RhoA/Ezrin-Radixin-Moesin 
      pathway and the expression of CD206. Moreover, H-iDCs released higher levels of 
      CXCL1, VEGF, CCL20, CXCL8, and CXCL10 but decreased levels of CCL2 and CCL18, 
      which predict a different ability to recruit neutrophils rather than monocytes 
      and create a proinflammatory and proangiogenic environment. By contrast, hypoxia 
      has no effect on DC maturation. Hypoxic mature DCs display a mature phenotype and 
      activate both allogeneic and specific T cells like normoxic mDCs. This study 
      provides the first demonstration that hypoxia inhibits antigen uptake by DCs and 
      profoundly changes the DC chemokine expression profile and may have a critical 
      role in DC differentiation, adaptation, and activation in inflamed tissues.
FAU - Elia, Angela Rita
AU  - Elia AR
AD  - Centre for Experimental Research and Medical Studies, San Giovanni Battista 
      Hospital, Torino, Italy.
FAU - Cappello, Paola
AU  - Cappello P
FAU - Puppo, Maura
AU  - Puppo M
FAU - Fraone, Tiziana
AU  - Fraone T
FAU - Vanni, Cristina
AU  - Vanni C
FAU - Eva, Alessandra
AU  - Eva A
FAU - Musso, Tiziana
AU  - Musso T
FAU - Novelli, Francesco
AU  - Novelli F
FAU - Varesio, Luigi
AU  - Varesio L
FAU - Giovarelli, Mirella
AU  - Giovarelli M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080825
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Antigens)
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (ETV5 protein, human)
RN  - 0 (Transcription Factors)
RN  - EC 3.6.5.2 (rho GTP-Binding Proteins)
SB  - IM
MH  - Antigens/*metabolism
MH  - Cell Differentiation/*physiology
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Chemokines/*metabolism
MH  - Cytokines/metabolism
MH  - DNA-Binding Proteins/metabolism
MH  - Dendritic Cells/*cytology/metabolism
MH  - Down-Regulation
MH  - Flow Cytometry
MH  - Humans
MH  - Hypoxia/*metabolism
MH  - Lymphocyte Activation
MH  - Macrophages/cytology/immunology/metabolism
MH  - Monocytes/cytology/immunology/metabolism
MH  - Phenotype
MH  - Phosphorylation
MH  - T-Lymphocytes/immunology/metabolism
MH  - Transcription Factors/metabolism
MH  - rho GTP-Binding Proteins/metabolism
EDAT- 2008/08/30 09:00
MHDA- 2009/01/23 09:00
CRDT- 2008/08/30 09:00
PHST- 2008/08/30 09:00 [pubmed]
PHST- 2009/01/23 09:00 [medline]
PHST- 2008/08/30 09:00 [entrez]
AID - jlb.0208082 [pii]
AID - 10.1189/jlb.0208082 [doi]
PST - ppublish
SO  - J Leukoc Biol. 2008 Dec;84(6):1472-82. doi: 10.1189/jlb.0208082. Epub 2008 Aug 
      25.