PMID- 18726426
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20091216
LR  - 20161020
IS  - 1006-9305 (Print)
IS  - 1006-9305 (Linking)
VI  - 44
IP  - 4
DP  - 2001 Aug
TI  - Three-dimensional structure of the Chinese Sacbrood bee virus.
PG  - 443-8
LID - 10.1007/BF02879612 [doi]
AB  - The RNA of Chinese Sacbrood Bee Virus (CSBV) was purified and used as template to 
      obtain a 1096 bp cDNA fragment by RT-PCR amplification. This DNA fragment was 
      cloned into pGEM-T Easy Vector for sequencing. Analyses of the sequenced CSBV RNA 
      fragment revealed a nucleotide sequence homology of 87.6% and a deduced 
      amino-acid sequence homology of 94.6% with that of the Sacbrood Virus (SBV), 
      indicating that CSBV is a different but highly homologous virus of SBV. The 
      three-dimensional (3D) structure of CSBV was determined at 2.5 nm resolution by 
      using electron cryo-microscopy (cryoEM) and computer reconstruction methods. The 
      3-D structure showed that the capsid has aT = 1 (or P = 3) icosahedral capsid 
      shell with a smooth surface. There were 12 pentons at its icosahedral vertices 
      (5-fold axes) and 132 holes penetrating the shell. The 3-D structure also 
      revealed densities corresponding to the CSBV genome, suggesting 
      icosahedrally-ordered RNA organization, a novel feature not previously reported 
      for any picornaviruses.
FAU - Zhang, J
AU  - Zhang J
AD  - Zhongshan University, Guangzhou, China.
FAU - Feng, J
AU  - Feng J
FAU - Liang, Y
AU  - Liang Y
FAU - Chen, D
AU  - Chen D
FAU - Zhou, Z H
AU  - Zhou ZH
FAU - Zhang, Q
AU  - Zhang Q
FAU - Lu, X
AU  - Lu X
LA  - eng
PT  - Journal Article
PL  - China
TA  - Sci China C Life Sci
JT  - Science in China. Series C, Life sciences
JID - 9611809
EDAT- 2008/08/30 09:00
MHDA- 2008/08/30 09:01
CRDT- 2008/08/30 09:00
PHST- 2001/01/11 00:00 [received]
PHST- 2008/08/30 09:00 [pubmed]
PHST- 2008/08/30 09:01 [medline]
PHST- 2008/08/30 09:00 [entrez]
AID - 10.1007/BF02879612 [doi]
PST - ppublish
SO  - Sci China C Life Sci. 2001 Aug;44(4):443-8. doi: 10.1007/BF02879612.