PMID- 18726687
OWN - NLM
STAT- MEDLINE
DCOM- 20090320
LR  - 20211020
IS  - 1573-6830 (Electronic)
IS  - 0272-4340 (Linking)
VI  - 29
IP  - 1
DP  - 2009 Feb
TI  - Hemolysate-induced expression of intercellular adhesion molecule-1 and monocyte 
      chemoattractant protein-1 expression in cultured brain microvascular endothelial 
      cells via through ROS-dependent NF-kappaB pathways.
PG  - 87-95
LID - 10.1007/s10571-008-9300-3 [doi]
AB  - In order to determine the possible effects of hemolysate on brain microvascular 
      endothelial cells (BMECs), we examined the effects of hemolysate on the 
      expression of intercellular adhesion molecule-1 (ICAM-1) and monocyte 
      chemoattractant protein-1 (MCP-1), generation of reactive oxygen species (ROS), 
      and NF-kappaB activation in rat BMECs. Hemolysate induced the expression of 
      ICAM-1 and MCP-1 in endothelial cells. In addition, hemolysate stimulated nuclear 
      translocation of the p65 subunit of NF-kappaB, and NF-kappaB DNA-binding activity 
      in BMECs. Furthermore, hemolysate increased ROS generation, and 
      hemolysate-induced ICAM-1and MCP-1 expression and NF-kappaB activation were 
      abrogated in the presence of the direct scavenger of ROS. Taken together, our 
      results indicate that hemolysate can induce inflammatory responses that increase 
      expression of ICAM-1 and MCP-1, through ROS-dependent NF-kappaB activation in 
      BMECs.
FAU - Lu, Hua
AU  - Lu H
AD  - Department of Neurosurgery, Jinling Hospital, Clinical School of Nanjing 
      University, Nanjing, China.
FAU - Shi, Ji-Xin
AU  - Shi JX
FAU - Zhang, Dong-Mei
AU  - Zhang DM
FAU - Shen, Jie
AU  - Shen J
FAU - Lin, Yi-Xing
AU  - Lin YX
FAU - Hang, Chun-Hua
AU  - Hang CH
FAU - Yin, Hong-Xia
AU  - Yin HX
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080826
PL  - United States
TA  - Cell Mol Neurobiol
JT  - Cellular and molecular neurobiology
JID - 8200709
RN  - 0 (Antioxidants)
RN  - 0 (Cell Extracts)
RN  - 0 (Chemokine CCL2)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
RN  - 0 (Reactive Oxygen Species)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Antioxidants/pharmacology
MH  - Brain/*cytology
MH  - Cell Extracts/*pharmacology
MH  - Cell Nucleus/drug effects/metabolism
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics/*metabolism
MH  - DNA/metabolism
MH  - Endothelial Cells/cytology/drug effects/*metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Intercellular Adhesion Molecule-1/genetics/*metabolism
MH  - NF-kappa B/*metabolism
MH  - Protein Binding/drug effects
MH  - Protein Transport/drug effects
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reactive Oxygen Species/*metabolism
MH  - Transcription, Genetic/drug effects
EDAT- 2008/08/30 09:00
MHDA- 2009/03/21 09:00
CRDT- 2008/08/30 09:00
PHST- 2008/04/21 00:00 [received]
PHST- 2008/07/31 00:00 [accepted]
PHST- 2008/08/30 09:00 [pubmed]
PHST- 2009/03/21 09:00 [medline]
PHST- 2008/08/30 09:00 [entrez]
AID - 10.1007/s10571-008-9300-3 [doi]
PST - ppublish
SO  - Cell Mol Neurobiol. 2009 Feb;29(1):87-95. doi: 10.1007/s10571-008-9300-3. Epub 
      2008 Aug 26.