PMID- 18729184
OWN - NLM
STAT- MEDLINE
DCOM- 20081010
LR  - 20220330
IS  - 1097-0215 (Electronic)
IS  - 0020-7136 (Linking)
VI  - 123
IP  - 10
DP  - 2008 Nov 15
TI  - Prognostic evaluation of epidermal fatty acid-binding protein and calcyphosine, 
      two proteins implicated in endometrial cancer using a proteomic approach.
PG  - 2377-83
LID - 10.1002/ijc.23808 [doi]
AB  - With the aim to translate the discovery from proteomic research into clinical 
      applications, we identified epidermal fatty acid-binding protein (E-FABP) and 
      calcyphosine (CAPS) by MALDI-Q-TOF MS and validated their overexpressions by 
      immunoblotting. Their expression statuses were examined by immunohistochemistry 
      in 39 normal endometrium, 29 endometrial intraepithelial neoplasia (EIN) and 84 
      endometrial cancer (EC) cases. We evaluated the correlations to the 
      clinicopathologic characteristics and determined whether these proteins had 
      prognostic significance. Expressions of E-FABP and CAPS were increased 2.64- and 
      2.18-fold in EC by immunoblotting. Immunoreactivity of both E-FABP and CAPS were 
      stronger in EC than in EIN or normal tissues (p < 0.001 and < 0.001). Stronger 
      immunoreactivity of E-FABP and CAPS were shown to present with poor 
      differentiation (p = 0.032 and 0.001), but no relevance was observed with staging 
      (p = 1.368 and 4.306). Survival analysis indicated that immunoreactivity of CAPS 
      was correlated to poor survival (p = 0.018), but E-FABP status appeared to be no 
      correlation to the clinical outcome of patients (p = 0.865). Multivariate 
      analysis indicated that CAPS might be an independent prognostic factor for 
      survival in patients with EC (p = 0.008). Results demonstrated the ubiquitous 
      overexpressions of E-FABP and CAPS in EC and the correlations to the 
      clinicopathologic parameters. CAPS might be a potential prognostic factor for 
      survival in patients with EC. The research pattern from proteomics to clinical 
      specimens would have widespread applications.
CI  - (c) 2008 Wiley-Liss, Inc.
FAU - Li, Zhengyu
AU  - Li Z
AD  - Department of Gynecology and Obstetrics, West China Second Hospital, Sichuan 
      University, Chengdu, People's Republic of China.
FAU - Huang, Canhua
AU  - Huang C
FAU - Bai, Shujun
AU  - Bai S
FAU - Pan, Xiaoling
AU  - Pan X
FAU - Zhou, Rong
AU  - Zhou R
FAU - Wei, Yuquan
AU  - Wei Y
FAU - Zhao, Xia
AU  - Zhao X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (CAPS protein, human)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Fatty Acid-Binding Proteins)
SB  - IM
MH  - Calcium-Binding Proteins/*metabolism
MH  - Epidermis/*metabolism
MH  - Fatty Acid-Binding Proteins/*metabolism
MH  - Humans
MH  - Prognosis
MH  - *Proteomics
EDAT- 2008/08/30 09:00
MHDA- 2008/10/11 09:00
CRDT- 2008/08/30 09:00
PHST- 2008/08/30 09:00 [pubmed]
PHST- 2008/10/11 09:00 [medline]
PHST- 2008/08/30 09:00 [entrez]
AID - 10.1002/ijc.23808 [doi]
PST - ppublish
SO  - Int J Cancer. 2008 Nov 15;123(10):2377-83. doi: 10.1002/ijc.23808.