PMID- 18729241
OWN - NLM
STAT- MEDLINE
DCOM- 20081125
LR  - 20191210
IS  - 0952-3499 (Print)
IS  - 0952-3499 (Linking)
VI  - 21
IP  - 5
DP  - 2008 Sep-Oct
TI  - Antibodies against RNA hydrolyze RNA and DNA.
PG  - 338-47
LID - 10.1002/jmr.906 [doi]
AB  - Immunization of animals with DNA leads to the production of anti-DNA antibodies 
      (Abs) demonstrating both DNase and RNase activities. It is currently not known 
      whether anti-RNA Abs can possess nuclease activities. In an attempt to address 
      this question, we have shown that immunization of three rabbits with complex of 
      RNA with methylated BSA (mBSA) stimulates production of IgGs with RNase and DNase 
      activities belonging to IgGs, while polyclonal Abs from three non-immunized 
      rabbits and three animals immunized with mBSA are catalytically inactive. 
      Affinity chromatography of IgGs from the sera of autoimmune (AI) patients on 
      DNA-cellulose usually demonstrates a number of fractions, all of which 
      effectively hydrolyze both DNA and RNA, while rabbit catalytic IgGs were 
      separated into Ab subfractions, some of which demonstrated only DNase activity, 
      while others hydrolyzed RNA faster than DNA. The enzymic properties of the RNase 
      and DNase IgGs from rabbits immunized with RNA distinguish them from all known 
      canonical RNases and DNases and DNA- and RNA-hydrolyzing abzymes (Abzs) from 
      patients with different AI diseases. In contrast to RNases and AI RNA-hydrolyzing 
      Abs, rabbit RNase IgGs catalyze only the first step of the hydrolysis reaction 
      but cannot hydrolyze the formed terminal 2',3'-cyclophosphate. The data indicate 
      that Abzs of AI patients hydrolyzing nucleic acids in part may be Abs against RNA 
      and its complexes with proteins.
CI  - Copyright (c) 2008 John Wiley & Sons, Ltd.
FAU - Krasnorutskii, Michael A
AU  - Krasnorutskii MA
AD  - Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the 
      Russian Academy of Sciences, pr. Lavrent'eva 8, Novosibirsk, Russia.
FAU - Buneva, Valentina N
AU  - Buneva VN
FAU - Nevinsky, Georgy A
AU  - Nevinsky GA
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Mol Recognit
JT  - Journal of molecular recognition : JMR
JID - 9004580
RN  - 0 (Antibodies, Antinuclear)
RN  - 0 (Antibodies, Catalytic)
RN  - 0 (DNA-cellulose)
RN  - 0 (Immunoglobulin G)
RN  - 63231-63-0 (RNA)
RN  - 9004-34-6 (Cellulose)
RN  - 9007-49-2 (DNA)
RN  - EC 3.1.- (Deoxyribonucleases)
RN  - EC 3.1.- (Ribonucleases)
SB  - IM
MH  - Animals
MH  - Antibodies, Antinuclear/pharmacology
MH  - Antibodies, Catalytic/metabolism/*pharmacology
MH  - Cellulose/analogs & derivatives/pharmacology
MH  - DNA/*metabolism/pharmacology
MH  - Deoxyribonucleases/metabolism
MH  - Hydrolysis/drug effects
MH  - Immunoglobulin G/metabolism/pharmacology
MH  - RNA/*immunology/*metabolism
MH  - Rabbits
MH  - Ribonucleases/metabolism
EDAT- 2008/08/30 09:00
MHDA- 2008/12/17 09:00
CRDT- 2008/08/30 09:00
PHST- 2008/08/30 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/08/30 09:00 [entrez]
AID - 10.1002/jmr.906 [doi]
PST - ppublish
SO  - J Mol Recognit. 2008 Sep-Oct;21(5):338-47. doi: 10.1002/jmr.906.