PMID- 1874953
OWN - NLM
STAT- MEDLINE
DCOM- 19910926
LR  - 20190510
IS  - 0268-1161 (Print)
IS  - 0268-1161 (Linking)
VI  - 6
IP  - 1
DP  - 1991 Jan
TI  - An investigation of the fate of cells transplanted orthotopically between 
      morulae/nascent blastocysts in the mouse.
PG  - 25-35
AB  - A technique has been devised for selectively replacing some inside cells (ICs) or 
      outside cells (OCs) of late morulae/nascent blastocysts with corresponding cells 
      from genetically dissimilar, synchronous embryos. The main purpose was to 
      determine whether the inner cell mass (ICM) contributes cells to the overlying 
      polar trophectoderm at any stage during the blastocyst phase of development. 
      Notwithstanding the high incidence and level of chimaerism in ICM derivatives of 
      post-implantation conceptuses obtained in the IC transplantation experiments, 
      trophoblast tissue was composed entirely of host cells in the majority of cases. 
      Even where a donor IC contribution to trophoblast was detected there were strong 
      grounds for suspecting that it was due to tissue contamination rather than 
      genuine chimaerism. Thus, not only did such contributions differ in both level 
      and distribution from those produced by transplantation of OCs but they also 
      varied markedly in frequency according to the day of gestation on which 
      conceptuses were dissected. The possibility that ICs regularly colonize the polar 
      trophectoderm but fail to persist there was excluded by the results of short-term 
      transplantation experiments using an in-situ genetic marker. These findings offer 
      no support for the hypothesis that the ICM serves as a population of stem cells 
      for the trophectoderm as well as the primitive endoderm and ectoderm during 
      normal development. The frequency of chimaerism was lower in OC than IC 
      transplantation experiments. Nevertheless, in a substantial proportion of 
      chimaeras, OCs colonized derivatives of the ICM. This is consistent with evidence 
      from other studies that some outside cells divide differentially at 5th cleavage 
      to produce an OC plus an IC rather than two OCs.
FAU - Gardner, R L
AU  - Gardner RL
AD  - Imperial Cancer Research Fund, Department of Zoology, Oxford, UK.
FAU - Nichols, J
AU  - Nichols J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
SB  - IM
MH  - Animals
MH  - *Cell Differentiation
MH  - Chimera
MH  - Embryonic and Fetal Development
MH  - Fetal Tissue Transplantation
MH  - Mice
MH  - Morula/*physiology
MH  - Trophoblasts/*physiology
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
CRDT- 1991/01/01 00:00
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
PHST- 1991/01/01 00:00 [entrez]
AID - 10.1093/oxfordjournals.humrep.a137254 [doi]
PST - ppublish
SO  - Hum Reprod. 1991 Jan;6(1):25-35. doi: 10.1093/oxfordjournals.humrep.a137254.