PMID- 18752275
OWN - NLM
STAT- MEDLINE
DCOM- 20090206
LR  - 20191210
IS  - 1554-527X (Electronic)
IS  - 0736-0266 (Linking)
VI  - 27
IP  - 2
DP  - 2009 Feb
TI  - Activation of rat nucleus pulposus cells by coculture with whole bone marrow 
      cells collected by the perfusion method.
PG  - 222-8
LID - 10.1002/jor.20740 [doi]
AB  - Cell proliferation and matrix synthesis were compared for rat nucleus pulposus 
      cells cocultured with mesenchymal stem cells (MSCs) or fresh whole bone marrow 
      cells (BMCs), harvested by the perfusion or aspiration methods. Nucleus pulposus 
      cells were isolated from tail intervertebral discs of F344/slc rats, and BMCs 
      were obtained from femora. Proteoglycan synthesis, DNA synthesis, and aggrecan 
      mRNA expression were measured. The level of transforming growth factor-beta in 
      supernatants from the culture system was also measured. Cell number, aggrecan 
      mRNA expression, and uptake of [(35)S]-sulfate and [(3)H]-thymidine by nucleus 
      pulposus cells cocultured with fresh whole BMCs all increased significantly 
      compared with nucleus pulposus cells cocultured with MSCs. TGF-beta secreted by 
      nucleus pulposus cells cocultured with fresh whole BMCs also significantly 
      increased when compared with cocultures with MSCs. The perfusion method was 
      superior to the aspiration method for preventing contamination of BMCs with 
      peripheral red blood cells and lymphocytes, which may cause an autoimmune 
      response in the disc. In conclusion, we suggest that fresh whole BMCs harvested 
      by the perfusion method are more effective for increasing the proliferative and 
      matrix synthesis capacity of nucleus pulposus cells.
FAU - Umeda, Masayuki
AU  - Umeda M
AD  - Department of Orthopedic Surgery, Kansai Medical University, Moriguchi-City, 
      Osaka, Japan.
FAU - Kushida, Taketoshi
AU  - Kushida T
FAU - Sasai, Kunihiko
AU  - Sasai K
FAU - Asada, Taku
AU  - Asada T
FAU - Oe, Kenichi
AU  - Oe K
FAU - Sakai, Daisuke
AU  - Sakai D
FAU - Mochida, Joji
AU  - Mochida J
FAU - Ikehara, Susumu
AU  - Ikehara S
FAU - Iida, Hirokazu
AU  - Iida H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Orthop Res
JT  - Journal of orthopaedic research : official publication of the Orthopaedic 
      Research Society
JID - 8404726
RN  - 0 (Acan protein, rat)
RN  - 0 (Aggrecans)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Proteoglycans)
SB  - IM
MH  - Aggrecans/genetics
MH  - Animals
MH  - Biopsy, Needle
MH  - Bone Marrow Cells/*cytology/*metabolism
MH  - CD4-Positive T-Lymphocytes/cytology
MH  - CD8-Positive T-Lymphocytes/cytology
MH  - Cell Count
MH  - Cell Division/physiology
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Erythrocytes/cytology
MH  - Intercellular Signaling Peptides and Proteins/metabolism
MH  - Intervertebral Disc/*cytology
MH  - Mesenchymal Stem Cells/*cytology/*metabolism
MH  - Perfusion
MH  - Proteoglycans/biosynthesis
MH  - Rats
MH  - Rats, Inbred F344
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tail
MH  - Tissue and Organ Harvesting/*methods
EDAT- 2008/08/30 09:00
MHDA- 2009/02/07 09:00
CRDT- 2008/08/30 09:00
PHST- 2008/08/30 09:00 [pubmed]
PHST- 2009/02/07 09:00 [medline]
PHST- 2008/08/30 09:00 [entrez]
AID - 10.1002/jor.20740 [doi]
PST - ppublish
SO  - J Orthop Res. 2009 Feb;27(2):222-8. doi: 10.1002/jor.20740.