PMID- 18753215
OWN - NLM
STAT- MEDLINE
DCOM- 20080930
LR  - 20200302
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 89
IP  - Pt 9
DP  - 2008 Sep
TI  - An infectious cDNA clone of a highly pathogenic porcine reproductive and 
      respiratory syndrome virus variant associated with porcine high fever syndrome.
PG  - 2075-2079
LID - 10.1099/vir.0.2008/001529-0 [doi]
AB  - Since May 2006, a so-called 'porcine high fever syndrome' (PHFS) has spread all 
      over China. The arterivirus porcine reproductive and respiratory syndrome virus 
      (PRRSV) was believed to be the main causative agent, although the involvement of 
      other pathogens was not formally excluded. The genome of a representative Chinese 
      PRRSV strain, named JX143, was sequenced and used to develop infectious cDNA 
      clones, pJX143 and pJX143M, with the latter containing an engineered MluI site 
      that served as a genetic marker. In various virological assays, the rescued 
      viruses, vJX143 and vJX143M, were indistinguishable from their parental virus. 
      Animal experiments showed that these recombinant viruses retained the high 
      pathogenicity and induced the typical clinical symptoms observed during PHFS 
      outbreaks. This is the first report describing infectious cDNA clones of this 
      highly pathogenic PRRSV. Our results unambiguously fulfil Koch's postulates and 
      define highly pathogenic PRRSV as the aetiological agent of PHFS in China.
FAU - Lv, Jian
AU  - Lv J
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, The Key Laboratory of Animal 
      Parasitology, Chinese Ministry of Agriculture, Shanghai 200232, PR China.
AD  - China Agricultural University, College of Biological Sciences, Beijing 100094, PR 
      China.
FAU - Zhang, Jianwu
AU  - Zhang J
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, The Key Laboratory of Animal 
      Parasitology, Chinese Ministry of Agriculture, Shanghai 200232, PR China.
FAU - Sun, Zhi
AU  - Sun Z
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, The Key Laboratory of Animal 
      Parasitology, Chinese Ministry of Agriculture, Shanghai 200232, PR China.
FAU - Liu, Weiquan
AU  - Liu W
AD  - China Agricultural University, College of Biological Sciences, Beijing 100094, PR 
      China.
FAU - Yuan, Shishan
AU  - Yuan S
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, The Key Laboratory of Animal 
      Parasitology, Chinese Ministry of Agriculture, Shanghai 200232, PR China.
LA  - eng
SI  - GENBANK/EF488048
SI  - GENBANK/EU708726
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Antibodies, Viral)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Cell Line
MH  - China/epidemiology
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - DNA, Viral/*genetics
MH  - Disease Outbreaks/veterinary
MH  - Fever/veterinary/virology
MH  - Molecular Sequence Data
MH  - Porcine Reproductive and Respiratory Syndrome/epidemiology/immunology/*virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/*genetics/immunology/*pathogenicity
MH  - Recombination, Genetic
MH  - Swine
MH  - Virus Cultivation
EDAT- 2008/08/30 09:00
MHDA- 2008/10/01 09:00
CRDT- 2008/08/30 09:00
PHST- 2008/08/30 09:00 [pubmed]
PHST- 2008/10/01 09:00 [medline]
PHST- 2008/08/30 09:00 [entrez]
AID - 10.1099/vir.0.2008/001529-0 [doi]
PST - ppublish
SO  - J Gen Virol. 2008 Sep;89(Pt 9):2075-2079. doi: 10.1099/vir.0.2008/001529-0.