PMID- 18753242
OWN - NLM
STAT- MEDLINE
DCOM- 20080930
LR  - 20200302
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 89
IP  - Pt 9
DP  - 2008 Sep
TI  - Genomic and host range studies of Maruca vitrata nucleopolyhedrovirus.
PG  - 2315-2330
LID - 10.1099/vir.0.2008/001412-0 [doi]
AB  - The complete genome of the Maruca vitrata nucleopolyhedrovirus (MaviNPV) isolated 
      from the legume pod borer, Maruca vitrata (Lepidoptera: Pyralidae), was 
      sequenced. It was found to be 111 953 bp in length, with an overall 39 % G+C 
      content, and contained 126 open reading frames (ORFs) encoding predicted proteins 
      of over 50 aa. The gene content and gene order of MaviNPV have the highest 
      similarity to those of Autographa californica multiple nucleopolyhedrovirus 
      (AcMNPV) and their shared homologous genes are 100 % collinear. In fact, MaviNPV 
      seems to be a mini-AcMNPV that is native to Taiwan and possesses a smaller genome 
      with fewer auxiliary genes than the AcMNPV type species. Except for one ORF 
      (Mv74), all of the MaviNPV ORFs have homologues in the AcMNPV genome. MaviNPV is 
      the first lepidopteran-specific baculovirus to lack homologues of vfgf and 
      odv-e66. In addition, MaviNPV lacks the baculovirus repeat ORF (bro) gene that 
      corresponds to AcMNPV ORF2. Five homologous regions (hrs) were located within the 
      MaviNPV genome, and these contained a total of 44 imperfect palindromes. 
      Phylogenetic analysis of the whole genome revealed that MaviNPV was separated 
      from the common ancestor of AcMNPV and Bombyx mori nucleopolyhedrovirus before 
      these two viral species diverged from each other. Moreover, replication of 
      MaviNPV in several cell lines and an egfp-MaviNPV infection assay revealed that 
      IPLB-LD-652Y cells are only partially permissive to MaviNPV, which supports our 
      conclusion that MaviNPV is a distinct species of the group I lepidopteran NPVs.
FAU - Chen, Yun-Ru
AU  - Chen YR
AD  - Department of Entomology, National Taiwan University, Taipei, Taiwan, ROC.
FAU - Wu, Chih-Yu
AU  - Wu CY
AD  - Department of Entomology, National Taiwan University, Taipei, Taiwan, ROC.
FAU - Lee, Song-Tay
AU  - Lee ST
AD  - Department of Biotechnology, Southern Taiwan University of Technology, No. 1 
      Nantai Street, Yung-Kang City, Tainan 710, Taiwan, ROC.
FAU - Wu, Yan-Jheng
AU  - Wu YJ
AD  - Department of Biotechnology, Southern Taiwan University of Technology, No. 1 
      Nantai Street, Yung-Kang City, Tainan 710, Taiwan, ROC.
FAU - Lo, Chu-Fang
AU  - Lo CF
AD  - Department of Zoology, National Taiwan University, Taipei, Taiwan, ROC.
FAU - Tsai, Meng-Feng
AU  - Tsai MF
AD  - Department of Molecular Biotechnology, Dayeh University, No. 112 Shanjiao Road, 
      Dacun, Changhua, Taiwan, ROC.
FAU - Wang, Chung-Hsiung
AU  - Wang CH
AD  - Department of Entomology, National Taiwan University, Taipei, Taiwan, ROC.
LA  - eng
SI  - GENBANK/EF125867
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
RN  - 0 (Viral Proteins)
RN  - 0 (Viral Structural Proteins)
RN  - 0 (p35 protein, Baculovirus)
SB  - IM
MH  - Animals
MH  - Baculoviridae/classification/genetics
MH  - Base Composition
MH  - Base Sequence
MH  - Cell Line
MH  - DNA Primers/genetics
MH  - DNA Replication/genetics
MH  - DNA, Viral/chemistry/genetics
MH  - Genome, Viral
MH  - Host-Pathogen Interactions
MH  - Molecular Sequence Data
MH  - Moths/*virology
MH  - Nucleopolyhedroviruses/classification/*genetics/isolation & 
      purification/pathogenicity
MH  - Open Reading Frames
MH  - Phylogeny
MH  - Species Specificity
MH  - Viral Proteins/genetics
MH  - Viral Structural Proteins/genetics
EDAT- 2008/08/30 09:00
MHDA- 2008/10/01 09:00
CRDT- 2008/08/30 09:00
PHST- 2008/08/30 09:00 [pubmed]
PHST- 2008/10/01 09:00 [medline]
PHST- 2008/08/30 09:00 [entrez]
AID - 10.1099/vir.0.2008/001412-0 [doi]
PST - ppublish
SO  - J Gen Virol. 2008 Sep;89(Pt 9):2315-2330. doi: 10.1099/vir.0.2008/001412-0.