PMID- 18753439
OWN - NLM
STAT- MEDLINE
DCOM- 20081028
LR  - 20220317
IS  - 0032-5791 (Print)
IS  - 0032-5791 (Linking)
VI  - 87
IP  - 9
DP  - 2008 Sep
TI  - Stronger in vitro phagocytosis by monocytes-macrophages is indicative of greater 
      pathogen clearance and antibody levels in vivo.
PG  - 1725-33
LID - 10.3382/ps.2007-00202 [doi]
AB  - Monocytes-macrophages are crucial players in specific and nonspecific immune 
      responses to protect organisms from invasion of bacteria or viruses. In this 
      study, monocytes in circulation from 2 lines of Silky and Starbro chickens with 
      different disease resistance were separated and cultured in vitro. After 
      identification with acridine orange (AO), Giemsa staining, and CD14 
      immunostaining, monocytes-macrophages were used for adherence and phagocytosis 
      test. The overall percentages of adherence of Silky monocytes was 1.5 times 
      greater than that of Starbro (P < 0.01), which were 26.85% +/- 8.24% and 18.34% 
      +/- 8.15%, respectively (mean +/- SD). The monocytes-macrophages phagocytic 
      index, phagocytic product, and percentage of phagocytosis in Silkies were greater 
      than in Star-bros, respectively. The difference of phagocytic index was 
      significant (P < 0.05), that is, 3.70 +/- 1.75 and 1.97 +/- 0.31, respectively 
      (mean +/- SD). Then, 20 Silkies were divided into 2 groups according to 
      phagocytic index: high phagocytic index (HPI) group and low phagocytic index 
      (LPI) group, to study the relationship between phagocytic activity in vitro and 
      pathogen clearance. After being challenged against Salmonella Pullorum C79-13, 
      the Silky birds with HPI produced a 3-fold greater level of specific antibodies 
      compared with those with LPI (P < 0.01), 50.21 +/- 6.67 and 16.85 +/- 4.52, 
      respectively (mean +/- SD). In contrast to LPI birds, HPI birds shed less 
      Salmonella Pullorum bacteria (P < 0.05), that is, 168.98 x 10(8) +/- 294.74 x 
      10(8) compared to 385.40 x 10(8) +/- 399.94 x 10(8) (mean +/- SD), and the 
      shedding peak of Salmonella Pullorum in the test span appeared 4 d earlier. These 
      results indicated that phagocytosis of monocytes-macrophages had strong effects 
      on antibody titer and bacteria shedding postchallenge, which could be used to 
      predict the disease resistance in animals.
FAU - Sun, S F
AU  - Sun SF
AD  - National Key Laboratory of Agricultural Biotechnology, Beijing, China.
FAU - Pan, Q Z
AU  - Pan QZ
FAU - Hui, X
AU  - Hui X
FAU - Zhang, B L
AU  - Zhang BL
FAU - Wu, H M
AU  - Wu HM
FAU - Li, H
AU  - Li H
FAU - Xu, W
AU  - Xu W
FAU - Zhang, Q
AU  - Zhang Q
FAU - Li, J Y
AU  - Li JY
FAU - Deng, X M
AU  - Deng XM
FAU - Chen, J W
AU  - Chen JW
FAU - Lian, Z X
AU  - Lian ZX
FAU - Li, N
AU  - Li N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Poult Sci
JT  - Poultry science
JID - 0401150
RN  - 0 (Antibodies, Bacterial)
SB  - IM
MH  - Animals
MH  - Antibodies, Bacterial/*blood
MH  - Cell Adhesion/physiology
MH  - Chickens/*immunology
MH  - Female
MH  - Host-Pathogen Interactions
MH  - Macrophages/cytology/*physiology
MH  - Monocytes/cytology/*physiology
MH  - Oviposition
MH  - Phagocytosis/physiology
MH  - Poultry Diseases/immunology/microbiology
MH  - Salmonella/immunology
MH  - Salmonella Infections, Animal/blood/*immunology
MH  - Time Factors
EDAT- 2008/08/30 09:00
MHDA- 2008/10/29 09:00
CRDT- 2008/08/30 09:00
PHST- 2008/08/30 09:00 [pubmed]
PHST- 2008/10/29 09:00 [medline]
PHST- 2008/08/30 09:00 [entrez]
AID - S0032-5791(19)39936-5 [pii]
AID - 10.3382/ps.2007-00202 [doi]
PST - ppublish
SO  - Poult Sci. 2008 Sep;87(9):1725-33. doi: 10.3382/ps.2007-00202.