PMID- 18755143
OWN - NLM
STAT- MEDLINE
DCOM- 20081030
LR  - 20211020
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 376
IP  - 1
DP  - 2008 Nov 7
TI  - Ryanodine receptor phosphorylation at Serine 2030, 2808 and 2814 in rat 
      cardiomyocytes.
PG  - 80-5
LID - 10.1016/j.bbrc.2008.08.084 [doi]
AB  - The cardiac ryanodine receptor (RyR) controls Ca2+ release from the sarcoplasmic 
      reticulum (SR) during excitation-contraction coupling. Three phosphorylation 
      sites have been identified: Serine-(S)2808, S2814 and recently S2030. We measured 
      phosphorylation with at least two different antibodies per site and demonstrate 
      that for S2808 results were highly antibody-dependent and two out of three S2808 
      antibodies did not accurately report phosphorylation level. The RyR was 
      substantially phosphorylated in quiescent rat cardiomyocytes at S2808 and less so 
      at S2814, but appeared to be unphosphorylated at S2030. Basal phosphorylation at 
      S2808/S2814 was maintained by a Ca2+ dependent kinase other than 
      Ca2+/Calmodulin-dependent kinase (CaMKII). During stimulation with Isoproterenol 
      S2808 was phosphorylated by protein kinase A (PKA) and S2814 was phosphorylated 
      by CaMKII. Phosphatase 1 appears to be the main phosphatase dephosphorylating 
      S2808/S2814, but phosphatase 2a may also dephosphorylate S2814. RyR 
      phosphorylation is complex, but important in understanding RyR functional 
      modulation.
FAU - Huke, Sabine
AU  - Huke S
AD  - Department of Physiology, Loyola University Chicago, Maywood, IL 60153, USA; 
      Department of Clinical Pharmacology, Vanderbilt University, Nashville, TN 37232, 
      USA.
FAU - Bers, Donald M
AU  - Bers DM
LA  - eng
GR  - HL-80101/HL/NHLBI NIH HHS/United States
GR  - P01 HL080101/HL/NHLBI NIH HHS/United States
GR  - R01 HL030077-23/HL/NHLBI NIH HHS/United States
GR  - R01 HL064724/HL/NHLBI NIH HHS/United States
GR  - HL-30077/HL/NHLBI NIH HHS/United States
GR  - R01 HL030077/HL/NHLBI NIH HHS/United States
GR  - P01 HL080101-02/HL/NHLBI NIH HHS/United States
GR  - R37 HL030077-27/HL/NHLBI NIH HHS/United States
GR  - R37 HL030077/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20080826
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Antibodies, Phospho-Specific)
RN  - 0 (Ryanodine Receptor Calcium Release Channel)
RN  - 17885-08-4 (Phosphoserine)
RN  - 452VLY9402 (Serine)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
RN  - EC 3.1.3.2 (Phosphoric Monoester Hydrolases)
RN  - L628TT009W (Isoproterenol)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Antibodies, Phospho-Specific/immunology
MH  - Base Sequence
MH  - Calcium/metabolism
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 2/antagonists & 
      inhibitors/metabolism
MH  - Cells, Cultured
MH  - Cyclic AMP-Dependent Protein Kinases/antagonists & inhibitors/metabolism
MH  - Isoproterenol/pharmacology
MH  - Myocytes, Cardiac/*metabolism
MH  - Phosphoric Monoester Hydrolases/metabolism
MH  - Phosphorylation/drug effects
MH  - Phosphoserine/immunology
MH  - Rats
MH  - Ryanodine Receptor Calcium Release Channel/*metabolism
MH  - Serine/*metabolism
PMC - PMC2581610
MID - NIHMS72842
EDAT- 2008/08/30 09:00
MHDA- 2008/10/31 09:00
PMCR- 2009/11/07
CRDT- 2008/08/30 09:00
PHST- 2008/08/16 00:00 [received]
PHST- 2008/08/20 00:00 [accepted]
PHST- 2008/08/30 09:00 [pubmed]
PHST- 2008/10/31 09:00 [medline]
PHST- 2008/08/30 09:00 [entrez]
PHST- 2009/11/07 00:00 [pmc-release]
AID - S0006-291X(08)01637-9 [pii]
AID - 10.1016/j.bbrc.2008.08.084 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2008 Nov 7;376(1):80-5. doi: 
      10.1016/j.bbrc.2008.08.084. Epub 2008 Aug 26.