PMID- 18755151
OWN - NLM
STAT- MEDLINE
DCOM- 20081027
LR  - 20131121
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 375
IP  - 4
DP  - 2008 Oct 31
TI  - Bone marrow stromal cells induce apoptosis of lymphoma cells in the presence of 
      IFNgamma and TNF by producing nitric oxide.
PG  - 666-70
LID - 10.1016/j.bbrc.2008.08.077 [doi]
AB  - Bone marrow stromal cells (BMSCs) have been shown to promote the growth and 
      survival of a wide variety of tumors. However, in the present study, we found 
      that BMSCs induced apoptosis of lymphoma cells in the presence of INFgamma and 
      TNF. IFNgamma and TNF dramatically induced the expression of inducible nitric 
      oxide synthase (iNOS) by BMSCs in culture, and BMSCs generated from iNOS knockout 
      mice did not induce apoptosis of lymphoma cells in the presence of IFNgamma and 
      TNF. In addition, we found that IFNgamma and TNF also increased IL-6 expression 
      by BMSCs, and anti-IL-6 further increased the killing of tumor cells by BMSCs. 
      Taken together, our findings indicate that BMSCs induce apoptosis of lymphoma 
      cells in the presence of IFNgamma and TNF, and that the proapoptotic effect of 
      BMSCs is mediated by nitric oxide. Our findings suggest a possibility to harness 
      this proapoptotic feature of BMSCs for the development of novel therapeutic 
      strategy to eliminate tumor cells, especially tumor cells in bone marrow.
FAU - Xu, Guangwu
AU  - Xu G
AD  - Department of Molecular Genetics, Microbiology and Immunology, Robert Wood 
      Johnson Medical School-University of Medicine and Dentistry of New Jersey, 661 
      Hoes Lane, Piscataway, NJ 08854, USA.
FAU - Zhang, Yingyu
AU  - Zhang Y
FAU - Zhang, Liying
AU  - Zhang L
FAU - Ren, Guangwen
AU  - Ren G
FAU - Shi, Yufang
AU  - Shi Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080826
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nos2 protein, mouse)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Bone Marrow Cells/*drug effects/enzymology
MH  - Cell Line, Tumor
MH  - Interferon-gamma/*pharmacology
MH  - Lymphoma/pathology/*therapy
MH  - Mice
MH  - Mice, Knockout
MH  - Nitric Oxide/*biosynthesis
MH  - Nitric Oxide Synthase Type II/genetics
MH  - Stromal Cells/drug effects/enzymology
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 2008/08/30 09:00
MHDA- 2008/10/28 09:00
CRDT- 2008/08/30 09:00
PHST- 2008/08/13 00:00 [received]
PHST- 2008/08/15 00:00 [accepted]
PHST- 2008/08/30 09:00 [pubmed]
PHST- 2008/10/28 09:00 [medline]
PHST- 2008/08/30 09:00 [entrez]
AID - S0006-291X(08)01621-5 [pii]
AID - 10.1016/j.bbrc.2008.08.077 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2008 Oct 31;375(4):666-70. doi: 
      10.1016/j.bbrc.2008.08.077. Epub 2008 Aug 26.