PMID- 18758299
OWN - NLM
STAT- MEDLINE
DCOM- 20081204
LR  - 20211020
IS  - 1556-1380 (Electronic)
IS  - 1556-0864 (Print)
IS  - 1556-0864 (Linking)
VI  - 3
IP  - 9
DP  - 2008 Sep
TI  - 3p22.1 and 10q22.3 deletions detected by fluorescence in situ hybridization 
      (FISH): a potential new tool for early detection of non-small cell lung Cancer 
      (NSCLC).
PG  - 979-84
LID - 10.1097/JTO.0b013e3181834f3a [doi]
AB  - BACKGROUND: Our objective was to study the feasibility of detecting chromosomal 
      deletions at 3p22.1 and 10q22.3 by fluorescent in situ hybridization (FISH) and 
      to examine their distribution in different areas of the airway in patients with 
      non-small cell lung cancer. METHODS: Brush biopsies from the mainstem bronchus on 
      the normal side contralateral to the tumor (NBB) and mainstem bronchus on the 
      tumor side (TBB) were obtained from 122 patients who underwent surgical 
      resection. Touch preparations from the tumor (TTP), normal lung parenchyma, and 
      bronchi adjacent to the tumor were also obtained. Two FISH assays using probes 
      complementary to 3p22.1 and 10q22.3 were used to detect deletions. RESULTS: NBB 
      showed a relatively low deletion rate of 3p22.1 and 10q22.3 compared with TTP (p 
      < 0.0001). TBB showed a significantly higher rate of deletions compared with NBB 
      but lower than TTP from the tumor (p < 0.05) for both 3p22.1 and 10q22.3. A 
      significantly higher deletion rate was seen at TTP compared with normal lung 
      parenchyma at both the 3p22.1 and 10 q22.3 (p < 0.0001). Correlations were seen 
      between the deletion rates of TTP and TBB at 3p22.1 (rho = 0.61, p < 0.0001) and 
      between TTP and bronchi adjacent to the tumor at 10q22.3 (rho = 0.64, p < 
      0.0001). CONCLUSION: Deletions of the 3p22.1 and 10q22.3 regions can be reliably 
      detected by FISH. As one progresses from the contralateral normal bronchus to the 
      bronchus on the side of tumor and the tumor itself, the percentage of chromosomal 
      deletions increases in a statistically significant fashion. This suggests that, 
      FISH analysis of bronchoscopic brushes may be useful for identifying patients at 
      high risk for developing non-small cell lung cancer.
FAU - Yendamuri, Sai
AU  - Yendamuri S
AD  - Department of Thoracic Surgery, Roswell Park Cancer Institute, Buffalo, New York, 
      USA.
FAU - Vaporciyan, Ara A
AU  - Vaporciyan AA
FAU - Zaidi, Tanweer
AU  - Zaidi T
FAU - Feng, Lei
AU  - Feng L
FAU - Fernandez, Ricardo
AU  - Fernandez R
FAU - Bekele, Nebiyou B
AU  - Bekele NB
FAU - Hofstetter, Wayne L
AU  - Hofstetter WL
FAU - Jiang, Feng
AU  - Jiang F
FAU - Mehran, Reza J
AU  - Mehran RJ
FAU - Rice, David C
AU  - Rice DC
FAU - Spitz, Margaret R
AU  - Spitz MR
FAU - Swisher, Stephen G
AU  - Swisher SG
FAU - Walsh, Garrett L
AU  - Walsh GL
FAU - Roth, Jack A
AU  - Roth JA
FAU - Katz, Ruth L
AU  - Katz RL
LA  - eng
GR  - P50 CA070907/CA/NCI NIH HHS/United States
GR  - R01 CA055769/CA/NCI NIH HHS/United States
GR  - P50CA70907/CA/NCI NIH HHS/United States
GR  - CA 55769/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Thorac Oncol
JT  - Journal of thoracic oncology : official publication of the International 
      Association for the Study of Lung Cancer
JID - 101274235
SB  - IM
MH  - Adenocarcinoma/diagnosis/genetics
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Bronchi/pathology
MH  - Carcinoma, Non-Small-Cell Lung/*diagnosis/genetics
MH  - Carcinoma, Squamous Cell/diagnosis/genetics
MH  - Cell Differentiation
MH  - *Chromosome Deletion
MH  - Chromosomes, Human, Pair 10/*genetics
MH  - Chromosomes, Human, Pair 3/*genetics
MH  - Cohort Studies
MH  - Early Diagnosis
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lung Neoplasms/*diagnosis/genetics
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Prognosis
MH  - Survival Rate
PMC - PMC3370669
MID - NIHMS381558
COIS- Disclosure: Drs. Katz and Jiang are holders of an issued USA patent (US Patent 
      Appl. No. 20060078885) for FISH probes to 3p22.1 and 10q22-23. The other authors 
      declare no conflict of interest.
EDAT- 2008/09/02 09:00
MHDA- 2008/12/17 09:00
PMCR- 2012/06/08
CRDT- 2008/09/02 09:00
PHST- 2008/09/02 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/09/02 09:00 [entrez]
PHST- 2012/06/08 00:00 [pmc-release]
AID - S1556-0864(15)30475-5 [pii]
AID - 10.1097/JTO.0b013e3181834f3a [doi]
PST - ppublish
SO  - J Thorac Oncol. 2008 Sep;3(9):979-84. doi: 10.1097/JTO.0b013e3181834f3a.