PMID- 18759875
OWN - NLM
STAT- MEDLINE
DCOM- 20081210
LR  - 20121115
IS  - 1600-0536 (Electronic)
IS  - 0105-1873 (Linking)
VI  - 59
IP  - 2
DP  - 2008 Aug
TI  - Proliferative responses in the local lymph node assay associated with concomitant 
      exposure to 1,4-phenylenediamine and methyldibromo glutaronitrile: evidence for 
      synergy?
PG  - 90-5
LID - 10.1111/j.1600-0536.2008.01349.x [doi]
AB  - BACKGROUND: A key consideration when undertaking risk assessments should be the 
      potential for synergy between contact allergens. Previously, this concept has 
      only been investigated during elicitation in contact allergic individuals. 
      OBJECTIVE: To determine whether there exists evidence for synergy between contact 
      allergens during the induction phase of skin sensitization using the mouse local 
      lymph node assay (LLNA) as a model system. METHOD: Proliferative responses in 
      draining lymph nodes were assessed with increasing concentrations of 
      1,4-phenylenediamine (PPD), methyldibromo glutaronitrile (MDBGN), and a 
      combination of PPD and MDBGN. RESULT: Data from each of two independent 
      experiments show that lymph node cell proliferation associated with combined 
      exposure to PPD and MDBGN was, in general, only modestly increased relative to 
      that predicted from a simple summation of their individual responses. 
      CONCLUSIONS: Although the increase in response is very modest, it does imply a 
      relationship between this combination of sensitizers that may not be simply 
      additive in terms of their ability to stimulate proliferative responses in 
      draining lymph nodes. The reproducibility of this observation should be confirmed 
      in future studies with additional pairs of contact allergens to ascertain whether 
      or not this represents evidence of synergy.
FAU - Jowsey, Ian R
AU  - Jowsey IR
AD  - Unilever Safety and Environmental Assurance Centre, Colworth Park, Sharnbrook, 
      Bedfordshire, MK44 1LQ, UK. ian.jowsey@smith-nephew.com
FAU - Basketter, David A
AU  - Basketter DA
FAU - Irwin, Anita
AU  - Irwin A
LA  - eng
PT  - Journal Article
PL  - England
TA  - Contact Dermatitis
JT  - Contact dermatitis
JID - 7604950
RN  - 0 (Allergens)
RN  - 0 (Nitriles)
RN  - 0 (Phenylenediamines)
RN  - 0 (Preservatives, Pharmaceutical)
RN  - U770QIT64J (4-phenylenediamine)
RN  - YX089CPS05 (1,2-dibromo-2,4-dicyanobutane)
SB  - IM
MH  - Allergens/*administration & dosage
MH  - Animals
MH  - Cell Proliferation/drug effects
MH  - Dermatitis, Allergic Contact/*etiology/pathology
MH  - Drug Interactions
MH  - Local Lymph Node Assay
MH  - Lymph Nodes/drug effects/pathology
MH  - Mice
MH  - Nitriles/*administration & dosage/toxicity
MH  - Phenylenediamines/*administration & dosage/toxicity
MH  - Preservatives, Pharmaceutical/*administration & dosage/toxicity
MH  - Risk Assessment
MH  - Skin/*drug effects
EDAT- 2008/09/02 09:00
MHDA- 2008/12/17 09:00
CRDT- 2008/09/02 09:00
PHST- 2008/09/02 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/09/02 09:00 [entrez]
AID - COD1349 [pii]
AID - 10.1111/j.1600-0536.2008.01349.x [doi]
PST - ppublish
SO  - Contact Dermatitis. 2008 Aug;59(2):90-5. doi: 10.1111/j.1600-0536.2008.01349.x.