PMID- 18760890
OWN - NLM
STAT- MEDLINE
DCOM- 20081217
LR  - 20151119
IS  - 0739-7240 (Print)
IS  - 0739-7240 (Linking)
VI  - 35
IP  - 4
DP  - 2008 Nov
TI  - Increased degradation of insulin-like growth factor-I in serum from feed-deprived 
      steers.
PG  - 343-51
LID - 10.1016/j.domaniend.2008.07.003 [doi]
AB  - Severe feed restriction decreases serum insulin-like growth factor I (IGF-I) 
      concentration in animals, and this decrease is thought to be due to reduced IGF-I 
      production in the liver. The objective of this study was to determine whether 
      feed deprivation also increases degradation of serum IGF-I and serum levels of 
      IGF binding protein 3 (IGFBP-3) and acid-labile subunit (ALS), which inhibit 
      IGF-I degradation and increase IGF-I retention in the blood by forming a ternary 
      complex with IGF-I, in cattle. Five steers had free access to pasture, and 
      another five were deprived of feed for 60 h. Serum concentration of IGF-I and 
      liver abundance of IGF-I mRNA at the end of the 60-h period were 50% and 80% 
      lower, respectively, in feed-deprived steers than in fed steers. Less 
      (125)I-labeled IGF-I remained intact after a 45-h incubation in sera of 
      feed-deprived steers than in sera of fed steers, suggesting that serum IGF-I is 
      more quickly degraded in feed-deprived animals. Serum levels of IGFBP-3 and ALS 
      were decreased by 40% and 30%, respectively, in feed-deprived steers compared 
      with fed steers. These decreases were associated with more than 50% reductions in 
      IGFBP-3 and ALS mRNA in the liver, the major source of serum IGFBP-3 and ALS. 
      Taken together, these results suggest that feed deprivation reduces serum 
      concentration of IGF-I in cattle not only by decreasing IGF-I gene expression in 
      the liver, but also by increasing IGF-I degradation and reducing IGF-I retention 
      in the blood through decreasing IGFBP-3 and ALS production in the liver.
FAU - Wu, Miaozong
AU  - Wu M
AD  - Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and 
      State University, Blacksburg, VA 24061-0306, United States.
FAU - Wang, Aihua
AU  - Wang A
FAU - Bernard, Gregory C
AU  - Bernard GC
FAU - Hall, John B
AU  - Hall JB
FAU - Beal, William E
AU  - Beal WE
FAU - Michael Akers, R
AU  - Michael Akers R
FAU - Boisclair, Yves R
AU  - Boisclair YR
FAU - Jiang, Honglin
AU  - Jiang H
LA  - eng
PT  - Journal Article
DEP - 20080821
PL  - United States
TA  - Domest Anim Endocrinol
JT  - Domestic animal endocrinology
JID - 8505191
RN  - 0 (Carrier Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (Insulin-Like Growth Factor Binding Protein 3)
RN  - 0 (Iodine Radioisotopes)
RN  - 0 (RNA, Messenger)
RN  - 0 (insulin-like growth factor binding protein, acid labile subunit)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - EC 3.1.- (Ribonucleases)
SB  - IM
MH  - Animals
MH  - Blotting, Western/veterinary
MH  - Carrier Proteins/blood/genetics
MH  - Cattle/*blood
MH  - Food Deprivation/*physiology
MH  - Glycoproteins/blood/genetics
MH  - Insulin-Like Growth Factor Binding Protein 3/blood/genetics
MH  - Insulin-Like Growth Factor I/genetics/*metabolism/pharmacokinetics
MH  - Iodine Radioisotopes
MH  - Liver/*metabolism
MH  - Male
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Radioimmunoassay
MH  - Ribonucleases/metabolism
EDAT- 2008/09/02 09:00
MHDA- 2008/12/18 09:00
CRDT- 2008/09/02 09:00
PHST- 2008/05/04 00:00 [received]
PHST- 2008/07/04 00:00 [revised]
PHST- 2008/07/05 00:00 [accepted]
PHST- 2008/09/02 09:00 [pubmed]
PHST- 2008/12/18 09:00 [medline]
PHST- 2008/09/02 09:00 [entrez]
AID - S0739-7240(08)00078-7 [pii]
AID - 10.1016/j.domaniend.2008.07.003 [doi]
PST - ppublish
SO  - Domest Anim Endocrinol. 2008 Nov;35(4):343-51. doi: 
      10.1016/j.domaniend.2008.07.003. Epub 2008 Aug 21.