PMID- 18762293
OWN - NLM
STAT- MEDLINE
DCOM- 20081112
LR  - 20081020
IS  - 1096-0953 (Electronic)
IS  - 0013-9351 (Linking)
VI  - 108
IP  - 2
DP  - 2008 Oct
TI  - Cellular uptake of lipoproteins and persistent organic compounds--an update and 
      new data.
PG  - 192-8
LID - 10.1016/j.envres.2008.07.019 [doi]
AB  - There are a number of interactions related to the transport of lipophilic 
      xenobiotic compounds in the blood stream of mammals. This paper will focus on the 
      interactions between lipoproteins and persistent organic pollutants (POPs) and 
      how these particles are taken up by cells. A number of POPs including the 
      pesticide p,p'-dichlorodiphenyltrichloroethane (DDT), and especially its 
      metabolite p,p'-dichlorodiphenyldichloroethene (DDE), interacts with nuclear 
      hormone receptors causing these to malfunction, which in turn results in a range 
      of deleterious health effects in humans. The aim of the present study was to 
      determine the role of lipoprotein receptors in mouse embryonic fibroblast (MEF) 
      cells in conjunction with uptake of DDT-lipoprotein complexes from supplemented 
      media in vitro. Uptake of DDT by MEF cells was investigated using MEF1 cells 
      carrying the receptors low-density lipoprotein receptor-related protein (LRP) and 
      low-density lipoprotein receptor (LDLR) present and MEF4 cells with no LRP and 
      LDLR expression. Cells were incubated together with the complex of low-density 
      lipoproteins (LDL) and [(14)C]DDT. The receptor function was further evaluated by 
      adding the 40kDa receptor-associated protein (RAP) which blocks receptor 
      activity. The results showed that [(14)C]DDT uptake was decreasing when the LDL 
      concentration was increasing. There was no strong evidence for a 
      receptor-mediated uptake of the [(14)C]DDT-lipoprotein complex. To conclude, DDT 
      travels in the blood stream and can cross cell membranes while being transported 
      as a DDT-lipoprotein complex. The lipoproteins do not need receptors to cross 
      cell membranes since passive diffusion constitutes a major passageway.
FAU - Hjelmborg, Philip Sebastian
AU  - Hjelmborg PS
AD  - Department of Environmental and Occupational medicine, Unit of Cellular and 
      Molecular Toxicology, Institute of Public Health, University of Aarhus, Aarhus, 
      Vennelyst Boulevard 6, Bygn 1260, 8000 Aarhus C, DK, Denmark.
FAU - Andreassen, Thomas Kjaergaard
AU  - Andreassen TK
FAU - Bonefeld-Jorgensen, Eva Cecilie
AU  - Bonefeld-Jorgensen EC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080830
PL  - Netherlands
TA  - Environ Res
JT  - Environmental research
JID - 0147621
RN  - 0 (Environmental Pollutants)
RN  - 0 (Lipoproteins)
RN  - 0 (Organic Chemicals)
RN  - 0 (Receptors, LDL)
SB  - IM
MH  - Animals
MH  - Biological Transport
MH  - Cell Culture Techniques
MH  - Cell Line
MH  - Environmental Pollutants/*pharmacokinetics
MH  - Fibroblasts/*drug effects/metabolism
MH  - Lipoproteins/*metabolism
MH  - Mice
MH  - Mice, Knockout
MH  - Organic Chemicals/*pharmacokinetics
MH  - Receptors, LDL/genetics
MH  - Ultracentrifugation
EDAT- 2008/09/03 09:00
MHDA- 2008/11/13 09:00
CRDT- 2008/09/03 09:00
PHST- 2007/11/01 00:00 [received]
PHST- 2008/05/13 00:00 [revised]
PHST- 2008/07/21 00:00 [accepted]
PHST- 2008/09/03 09:00 [pubmed]
PHST- 2008/11/13 09:00 [medline]
PHST- 2008/09/03 09:00 [entrez]
AID - S0013-9351(08)00152-7 [pii]
AID - 10.1016/j.envres.2008.07.019 [doi]
PST - ppublish
SO  - Environ Res. 2008 Oct;108(2):192-8. doi: 10.1016/j.envres.2008.07.019. Epub 2008 
      Aug 30.