PMID- 18764872
OWN - NLM
STAT- MEDLINE
DCOM- 20081223
LR  - 20211028
IS  - 1462-2920 (Electronic)
IS  - 1462-2912 (Linking)
VI  - 10
IP  - 12
DP  - 2008 Dec
TI  - Multiple bacterial symbionts in two species of co-occurring gutless oligochaete 
      worms from Mediterranean sea grass sediments.
PG  - 3404-16
LID - 10.1111/j.1462-2920.2008.01728.x [doi]
AB  - Gutless oligochaete worms are found worldwide in the pore waters of marine 
      sediments and live in symbiosis with chemoautotrophic sulfur-oxidizing bacteria. 
      In the Mediterranean, two species of gutless oligochaete worms, Olavius 
      algarvensis and O. ilvae, co-occur in sediments around sea grass beds. These 
      sediments have extremely low sulfide concentrations (< 1 microM), raising the 
      question if O. ilvae, as shown previously for O. algarvensis, also harbours 
      sulfate-reducing symbionts that provide its sulfur-oxidizing symbionts with 
      reduced sulfur compounds. In this study, we used fluorescence in situ 
      hybridization (FISH) and comparative sequence analysis of genes for 16S rRNA, 
      sulfur metabolism (aprA and dsrAB), and autotrophic carbon fixation (cbbL) to 
      examine the microbial community of O. ilvae and re-examine the O. algarvensis 
      symbiosis. In addition to the four previously described symbionts of O. 
      algarvensis, in this study a fifth symbiont belonging to the Spirochaetes was 
      found in these hosts. The symbiotic community of O. ilvae was similar to that of 
      O. algarvensis and also included two gammaproteobacterial sulfur oxidizers and 
      two deltaproteobacterial sulfate reducers, but not a spirochete. The phylogenetic 
      and metabolic similarity of the symbiotic communities in these two co-occurring 
      host species that are not closely related to each other indicates that syntrophic 
      sulfur cycling provides a strong selective advantage to these worms in their 
      sulfide-poor environment.
FAU - Ruehland, Caroline
AU  - Ruehland C
AD  - Symbiosis Group, Max Planck Institute for Marine Microbiology, Celsiusstrasse 1, 
      D-28359 Bremen, Germany.
FAU - Blazejak, Anna
AU  - Blazejak A
FAU - Lott, Christian
AU  - Lott C
FAU - Loy, Alexander
AU  - Loy A
FAU - Erseus, Christer
AU  - Erseus C
FAU - Dubilier, Nicole
AU  - Dubilier N
LA  - eng
GR  - P 18836/FWF_/Austrian Science Fund FWF/Austria
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080901
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 70FD1KFU70 (Sulfur)
SB  - IM
MH  - Animals
MH  - Bacteria/*classification/*isolation & purification
MH  - Bacterial Physiological Phenomena
MH  - Bacterial Proteins/genetics
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - Genes, rRNA
MH  - Geologic Sediments
MH  - In Situ Hybridization, Fluorescence
MH  - Mediterranean Sea
MH  - Molecular Sequence Data
MH  - Oligochaeta/*microbiology/physiology
MH  - Phylogeny
MH  - Poaceae
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Nucleic Acid
MH  - Sulfur/metabolism
MH  - *Symbiosis
EDAT- 2008/09/04 09:00
MHDA- 2008/12/24 09:00
CRDT- 2008/09/04 09:00
PHST- 2008/09/04 09:00 [pubmed]
PHST- 2008/12/24 09:00 [medline]
PHST- 2008/09/04 09:00 [entrez]
AID - EMI1728 [pii]
AID - 10.1111/j.1462-2920.2008.01728.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2008 Dec;10(12):3404-16. doi: 
      10.1111/j.1462-2920.2008.01728.x. Epub 2008 Sep 1.