PMID- 18765002
OWN - NLM
STAT- MEDLINE
DCOM- 20081031
LR  - 20190917
IS  - 1472-6491 (Electronic)
IS  - 1472-6483 (Linking)
VI  - 17
IP  - 3
DP  - 2008 Sep
TI  - Increased sperm ubiquitination correlates with abnormal chromatin integrity.
PG  - 324-30
AB  - Ubiquitin, a 8.5 kDa peptide that marks other proteins for proteasomal 
      degradation, tags defective spermatozoa during epididymal passage and is proposed 
      as a biomarker for sperm quality. The present study was designed to evaluate the 
      relationships between sperm ubiquitination, sperm chromatin integrity and semen 
      parameters. Semen samples from 63 couples were collected and analysed according 
      to World Health Organization criteria. Each sample was evaluated for sperm 
      ubiquitination by the direct immunofluorescence method, using anti-ubiquitin 
      antibodies. Chromatin integrity of the same samples was analysed using acridine 
      orange (AO) and toluidine blue (TB) tests. A positive correlation was found 
      between ubiquitinated spermatozoa and the percentage of spermatozoa with abnormal 
      chromatin (AO: r = 0.58, P < 0.001 and TB: r = 0.48, P < 0.001). Negative 
      correlations were obtained between sperm ubiquitination and: sperm count (r = 
      -0.2, P = 0.048), sperm morphology (r = -0.36, P = 0.003), rapidly progressive 
      motility (r = -0.25, P = 0.044) and slow progressive motility (r = -0.28, P = 
      0.022). Sperm ubiquitination was positively correlated with the percentage of 
      immotile spermatozoa. These results show that among semen parameters, chromatin 
      abnormality is more closely associated with sperm ubiquitination and further 
      validate sperm ubiquitination as a suitable marker for sperm quality.
FAU - Hodjat, M
AU  - Hodjat M
AD  - Reproductive Biotechnology Research Centre, Avicenna Research Institute, Evin, 
      Tehran, Iran.
FAU - Akhondi, M A
AU  - Akhondi MA
FAU - Al-Hasani, S
AU  - Al-Hasani S
FAU - Mobaraki, M
AU  - Mobaraki M
FAU - Sadeghi, M R
AU  - Sadeghi MR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Reprod Biomed Online
JT  - Reproductive biomedicine online
JID - 101122473
RN  - 0 (Chromatin)
RN  - 15XUH0X66N (Tolonium Chloride)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Chromatin/*ultrastructure
MH  - Humans
MH  - Infertility, Male/etiology/metabolism
MH  - Male
MH  - Sperm Count
MH  - Sperm Motility
MH  - Spermatozoa/*metabolism
MH  - Tolonium Chloride
MH  - *Ubiquitination
EDAT- 2008/09/04 09:00
MHDA- 2008/11/01 09:00
CRDT- 2008/09/04 09:00
PHST- 2008/09/04 09:00 [pubmed]
PHST- 2008/11/01 09:00 [medline]
PHST- 2008/09/04 09:00 [entrez]
AID - S1472-6483(10)60215-5 [pii]
AID - 10.1016/s1472-6483(10)60215-5 [doi]
PST - ppublish
SO  - Reprod Biomed Online. 2008 Sep;17(3):324-30. doi: 10.1016/s1472-6483(10)60215-5.