PMID- 18767064
OWN - NLM
STAT- MEDLINE
DCOM- 20091102
LR  - 20090826
IS  - 1552-4965 (Electronic)
IS  - 1549-3296 (Linking)
VI  - 91
IP  - 1
DP  - 2009 Oct
TI  - Effect of carbon nanotubes on cellular functions in vitro.
PG  - 132-9
LID - 10.1002/jbm.a.32203 [doi]
AB  - Carbon nanotubes (CNTs) have been shown to affect cell behavior. But how and why 
      the CNTs affect potential differentiation of the attached cells has not been 
      largely known. In this study, multiwalled carbon nanotubes (MWNTs) and graphite 
      (GP) were pressed as compacts. Higher ability of CNTs to adsorb proteins, 
      compared with GP, was shown. Myoblastic mouse cells (C2C12) were cultured and the 
      cell responses to the two kinds of compacts were compared in vitro. Meanwhile, we 
      used cell culture on the culture plate as a control. During the conventional 
      culture, significantly better cell attachment, proliferation, and differentiation 
      of cells on the MWNTs were found. To confirm the hypothesis that the larger 
      amount of protein adsorbed on the CNTs was crucial for this, we made the compacts 
      adsorb more proteins in culture medium with 50% fetal bovine serum (FBS) before 
      cell culture. With the adsorption of the proteins in advance, the increments of 
      the total-protein/DNA and alkaline phosphatase (ALP)/DNA for the MWNTs was 
      respectively as about 11 times and 18 times as the increments of those for GP and 
      the control at both day 4 and day 7. Therefore, the CNTs might induce cellular 
      functions by adsorbing more proteins, which indicated that the CNTs might be a 
      candidate for scaffold material for tissue engineering.
FAU - Li, Xiaoming
AU  - Li X
AD  - Department of Biomedical Materials and Engineering, Graduate School of Dental 
      Medicine, Hokkaido University, Sapporo 060-8586, Japan. x.m.li@hotmail.com
FAU - Gao, Hong
AU  - Gao H
FAU - Uo, Motohiro
AU  - Uo M
FAU - Sato, Yoshinori
AU  - Sato Y
FAU - Akasaka, Tsukasa
AU  - Akasaka T
FAU - Feng, Qingling
AU  - Feng Q
FAU - Cui, Fuzhai
AU  - Cui F
FAU - Liu, Xinhui
AU  - Liu X
FAU - Watari, Fumio
AU  - Watari F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 0 (Nanotubes, Carbon)
RN  - 0 (Proteins)
RN  - 7782-42-5 (Graphite)
SB  - IM
MH  - Adsorption
MH  - Animals
MH  - Cattle
MH  - Cell Adhesion
MH  - Cell Differentiation
MH  - Cell Line
MH  - Cell Proliferation
MH  - Graphite/*chemistry
MH  - Mice
MH  - Myoblasts/*cytology
MH  - Nanotubes, Carbon/*chemistry
MH  - Proteins/*chemistry
MH  - Serum/chemistry
EDAT- 2008/09/04 09:00
MHDA- 2009/11/03 06:00
CRDT- 2008/09/04 09:00
PHST- 2008/09/04 09:00 [pubmed]
PHST- 2009/11/03 06:00 [medline]
PHST- 2008/09/04 09:00 [entrez]
AID - 10.1002/jbm.a.32203 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2009 Oct;91(1):132-9. doi: 10.1002/jbm.a.32203.