PMID- 18767694
OWN - NLM
STAT- MEDLINE
DCOM- 20090122
LR  - 20190715
IS  - 0013-936X (Print)
IS  - 0013-936X (Linking)
VI  - 42
IP  - 16
DP  - 2008 Aug 15
TI  - Bacterial communities on electron-beam Pt-deposited electrodes in a mediator-less 
      microbial fuel cell.
PG  - 6243-9
AB  - The content of a bacterial consortium found on an electron beam (e-beam) 
      Pt-deposited electrode in a mediator-less microbial fuel cell (MFC) using glucose 
      and glutamate as fuel is reported in this paper. The e-beam Pt-deposited 
      electrode and electrochemically active bacteria (EAB) consortium were developed 
      to improve the mediator-less MFC performance. Denaturing gradient gel 
      electrophoresis (DGGE), restriction fragment length polymorphism (RFLP), and 16S 
      rRNA sequencing were used to identify the EAB consortia. Sequencing results 
      showed that clone ASP-31 was predominant and was similar to Aeromonas hydrophila, 
      an Fe(III)-reducing and EAB. The phylogenetic tree analysis disclosed the 
      presence of gamma-proteobacteria groups such as Aeromonas genus, Enterobacter 
      asburiae, and Klebsiella oxytoca. These results suggest that MFC performance of 
      the e-beam Pt-deposited electrode with Aeromonas genus consortia dominated by A. 
      hydrophila was higher than other MFCs within a short period. With the e-beam 
      Pt-deposited electrode and Aeromonas genus consortia in the mediator-less MFC, it 
      is possible to increase the efficiency of electron transfer between the bacteria 
      and the electrode.
FAU - Park, Ho Il
AU  - Park HI
AD  - Department of Electrical and Computer Engineering, University of Pittsburgh, 
      Pittsburgh, Pennsylvania 15261, USA.
FAU - Sanchez, David
AU  - Sanchez D
FAU - Cho, Sung Kwon
AU  - Cho SK
FAU - Yun, Minhee
AU  - Yun M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Environ Sci Technol
JT  - Environmental science & technology
JID - 0213155
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 49DFR088MY (Platinum)
RN  - 7440-44-0 (Carbon)
SB  - IM
MH  - Bacteria/genetics/*metabolism
MH  - *Bioelectric Energy Sources
MH  - Carbon/chemistry
MH  - DNA, Bacterial/genetics/isolation & purification
MH  - Electrochemistry
MH  - *Electrodes
MH  - *Electrons
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Microscopy, Electron, Scanning
MH  - Paper
MH  - Phylogeny
MH  - Platinum/*chemistry
MH  - Polymerase Chain Reaction
MH  - RNA, Ribosomal, 16S/genetics
EDAT- 2008/09/05 09:00
MHDA- 2009/01/23 09:00
CRDT- 2008/09/05 09:00
PHST- 2008/09/05 09:00 [pubmed]
PHST- 2009/01/23 09:00 [medline]
PHST- 2008/09/05 09:00 [entrez]
AID - 10.1021/es8006468 [doi]
PST - ppublish
SO  - Environ Sci Technol. 2008 Aug 15;42(16):6243-9. doi: 10.1021/es8006468.