PMID- 18767823
OWN - NLM
STAT- MEDLINE
DCOM- 20081028
LR  - 20161124
IS  - 0743-7463 (Print)
IS  - 0743-7463 (Linking)
VI  - 24
IP  - 19
DP  - 2008 Oct 7
TI  - Buried, covalently attached RGD peptide motifs in poly(methacrylic acid) brush 
      layers: the effect of brush structure on cell adhesion.
PG  - 10996-1002
LID - 10.1021/la800999y [doi]
AB  - Iniferter-mediated surface-initiated photopolymerization was used to graft 
      poly(methacrylic acid) (PMAA) brush layers obtained from surface-attached 
      iniferters in self-assembled monolayers to a gold surface. The tethered chains 
      were subsequently functionalized with the cell-adhesive arginine-glycine-aspartic 
      acid (RGD) motif. The modified brushes were extended by reinitiating the 
      polymerization to obtain an additional layer of PMAA, thereby burying the 
      peptide-functionalized segments inside the brush structure. Contact angle 
      measurements and Fourier transform infrared (FTIR) spectroscopy were employed to 
      characterize the wettability and the chemical properties of these platforms. Time 
      of flight secondary ion mass spectroscopy (TOF-SIMS) measurements were performed 
      to monitor the chemical composition of the polymer layer as a function of the 
      distance to the gold surface and obtain information concerning the depth of the 
      RGD motifs inside the brush structure. The brush thickness was evaluated as a 
      function of the polymerization (i.e., UV-irradiation) time with atomic force 
      microscopy (AFM) and ellipsometry. Cell adhesion tests employing human 
      osteoblasts were performed on substrates with the RGD peptides exposed at the 
      surface as well as covered by a PMAA top brush layer. Immunofluorescence studies 
      demonstrated a variation of the cell morphology as a function of the position of 
      the peptide units along the grafted chains.
FAU - Navarro, Melba
AU  - Navarro M
AD  - Materials Science and Technology of Polymers, MESA Institute for Nanotechnology, 
      University of Twente, P.O. Box 217, NL-7500 AE Enschede, The Netherlands.
FAU - Benetti, Edmondo M
AU  - Benetti EM
FAU - Zapotoczny, Szczepan
AU  - Zapotoczny S
FAU - Planell, Josep A
AU  - Planell JA
FAU - Vancso, G Julius
AU  - Vancso GJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080904
PL  - United States
TA  - Langmuir
JT  - Langmuir : the ACS journal of surfaces and colloids
JID - 9882736
RN  - 0 (Oligopeptides)
RN  - 0 (Polymethacrylic Acids)
RN  - 78VO7F77PN (arginyl-glycyl-aspartic acid)
SB  - IM
MH  - Cell Adhesion
MH  - Cell Line
MH  - Humans
MH  - Molecular Structure
MH  - Oligopeptides/*chemistry
MH  - Polymethacrylic Acids/*chemistry
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Surface Properties
EDAT- 2008/09/05 09:00
MHDA- 2008/10/29 09:00
CRDT- 2008/09/05 09:00
PHST- 2008/09/05 09:00 [pubmed]
PHST- 2008/10/29 09:00 [medline]
PHST- 2008/09/05 09:00 [entrez]
AID - 10.1021/la800999y [doi]
PST - ppublish
SO  - Langmuir. 2008 Oct 7;24(19):10996-1002. doi: 10.1021/la800999y. Epub 2008 Sep 4.