PMID- 18768155
OWN - NLM
STAT- MEDLINE
DCOM- 20090204
LR  - 20211020
IS  - 0378-5955 (Print)
IS  - 0378-5955 (Linking)
VI  - 245
IP  - 1-2
DP  - 2008 Nov
TI  - Over-expression of BDNF by adenovirus with concurrent electrical stimulation 
      improves cochlear implant thresholds and survival of auditory neurons.
PG  - 24-34
LID - 10.1016/j.heares.2008.08.005 [doi]
AB  - The survival of the auditory nerve in cases of sensorineural hearing loss is 
      believed to be a major factor in effective cochlear implant function. The current 
      study assesses two measures of cochlear implant thresholds following a 
      post-deafening treatment intended to halt auditory nerve degeneration. We used an 
      adenoviral construct containing a gene insert for brain-derived neurotrophic 
      factor (BDNF), a construct that has previously been shown to promote neuronal 
      survival in a number of biological systems. We implanted ototoxically deafened 
      guinea pigs with a multichannel cochlear implant and delivered a single 
      inoculation of an adenovirus suspension coding for BDNF (Ad.BDNF) into the scala 
      tympani at the time of implantation. Thresholds to electrical stimulation were 
      assessed both psychophysically and electrophysiologically over a period of 80 
      days. Spiral ganglion cell survival was analyzed at the 80 days time point. 
      Compared to the control group, the Ad.BDNF treated group had lower psychophysical 
      and electrophysiological thresholds as well as higher survival of spiral ganglion 
      cells. Electrophysiological, but not psychophysical, thresholds correlated well 
      with the density of spiral ganglion cells. These results indicate that the 
      changes in the anatomy of the auditory nerve induced by the combination of 
      Ad.BDNF inoculation and the electrical stimulation used for testing improved 
      functional measures of CI performance.
FAU - Chikar, Jennifer A
AU  - Chikar JA
AD  - Kresge Hearing Research Institute, Department of Otolaryngology, University of 
      Michigan Medical School, Ann Arbor, MI 48109-5616, United States. 
      jchikar@umich.edu
FAU - Colesa, Deborah J
AU  - Colesa DJ
FAU - Swiderski, Donald L
AU  - Swiderski DL
FAU - Di Polo, Adriana
AU  - Di Polo A
FAU - Raphael, Yehoash
AU  - Raphael Y
FAU - Pfingst, Bryan E
AU  - Pfingst BE
LA  - eng
GR  - P30-DC05188/DC/NIDCD NIH HHS/United States
GR  - R01 DC003389/DC/NIDCD NIH HHS/United States
GR  - R01 DC005401/DC/NIDCD NIH HHS/United States
GR  - P30 DC005188/DC/NIDCD NIH HHS/United States
GR  - T32 NS076401/NS/NINDS NIH HHS/United States
GR  - T32-DC00011/DC/NIDCD NIH HHS/United States
GR  - R01 DC001634-13/DC/NIDCD NIH HHS/United States
GR  - R01-DC05401/DC/NIDCD NIH HHS/United States
GR  - T32 DC000011/DC/NIDCD NIH HHS/United States
GR  - R01 DC005401-03/DC/NIDCD NIH HHS/United States
GR  - R01-DC03389/DC/NIDCD NIH HHS/United States
GR  - R01 DC001634/DC/NIDCD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20080819
PL  - Netherlands
TA  - Hear Res
JT  - Hearing research
JID - 7900445
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Adenoviridae/genetics
MH  - Animals
MH  - Auditory Threshold/*physiology
MH  - Brain-Derived Neurotrophic Factor/*genetics/*physiology
MH  - Cell Survival
MH  - *Cochlear Implants
MH  - Cochlear Nerve/cytology/*physiology
MH  - Electric Stimulation Therapy
MH  - Electrophysiology
MH  - Evoked Potentials, Auditory, Brain Stem
MH  - Gene Expression
MH  - Genetic Vectors
MH  - Guinea Pigs
MH  - Hearing Loss, Sensorineural/physiopathology/therapy
MH  - Humans
MH  - Male
MH  - Psychoacoustics
MH  - Recombinant Proteins/genetics
PMC - PMC2654221
MID - NIHMS80127
EDAT- 2008/09/05 09:00
MHDA- 2009/02/05 09:00
PMCR- 2009/11/01
CRDT- 2008/09/05 09:00
PHST- 2008/05/16 00:00 [received]
PHST- 2008/08/07 00:00 [revised]
PHST- 2008/08/12 00:00 [accepted]
PHST- 2008/09/05 09:00 [pubmed]
PHST- 2009/02/05 09:00 [medline]
PHST- 2008/09/05 09:00 [entrez]
PHST- 2009/11/01 00:00 [pmc-release]
AID - S0378-5955(08)00175-5 [pii]
AID - 10.1016/j.heares.2008.08.005 [doi]
PST - ppublish
SO  - Hear Res. 2008 Nov;245(1-2):24-34. doi: 10.1016/j.heares.2008.08.005. Epub 2008 
      Aug 19.