PMID- 18768197
OWN - NLM
STAT- MEDLINE
DCOM- 20081106
LR  - 20081020
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 380
IP  - 2
DP  - 2008 Oct 25
TI  - Identification of virulence determinants of porcine reproductive and respiratory 
      syndrome virus through construction of chimeric clones.
PG  - 371-8
LID - 10.1016/j.virol.2008.07.030 [doi]
AB  - In order to determine virulence associated genes in porcine reproductive and 
      respiratory syndrome virus (PRRSV), a series of chimeric viruses were generated 
      where specific genomic regions of a highly virulent PRRSV infectious clone (FL12) 
      were replaced with their counterparts of an attenuated vaccine strain (Prime 
      Pac). Initial genome-wide scanning using a sow reproductive failure model 
      indicated that non-structural (ORF 1a and 1b) and structural (ORF2-7) genomic 
      regions appear to be sites where virulence determinants of PRRSV may reside. 
      These results thus confirm the multigenic character of PRRSV virulence. 
      Additional chimeras containing each individual structural ORFs (2 through 7) of 
      Prime Pac and ORF5 of Neb-1 (parental strain of Prime Pac) within the FL12 
      backbone were generated and tested individually for further mapping of virulence 
      determinants. Our results allow to conclude that NSP3-8 and ORF5 are the location 
      of major virulence determinants, while other virulence determinants may also be 
      contained in NSP1-3, NSP10-12 and ORF2.
FAU - Kwon, Byungjoon
AU  - Kwon B
AD  - Department of Veterinary and Biomedical Sciences and Nebraska Center for 
      Virology, University of Nebraska-Lincoln, 111 K. Morrison Life Sc Res Center, 
      4240 Fair Street, East Campus, Lincoln, NE 68583-0900, USA.
FAU - Ansari, Israrul H
AU  - Ansari IH
FAU - Pattnaik, Asit K
AU  - Pattnaik AK
FAU - Osorio, Fernando A
AU  - Osorio FA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080902
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Viral Proteins)
RN  - 0 (Virulence Factors)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/growth & 
      development/*pathogenicity
MH  - Swine
MH  - Viral Proteins/genetics/*physiology
MH  - Virulence Factors/genetics/*physiology
EDAT- 2008/09/05 09:00
MHDA- 2008/11/07 09:00
CRDT- 2008/09/05 09:00
PHST- 2008/06/16 00:00 [received]
PHST- 2008/07/25 00:00 [revised]
PHST- 2008/07/26 00:00 [accepted]
PHST- 2008/09/05 09:00 [pubmed]
PHST- 2008/11/07 09:00 [medline]
PHST- 2008/09/05 09:00 [entrez]
AID - S0042-6822(08)00490-X [pii]
AID - 10.1016/j.virol.2008.07.030 [doi]
PST - ppublish
SO  - Virology. 2008 Oct 25;380(2):371-8. doi: 10.1016/j.virol.2008.07.030. Epub 2008 
      Sep 2.