PMID- 18768856
OWN - NLM
STAT- MEDLINE
DCOM- 20081118
LR  - 20211020
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 181
IP  - 6
DP  - 2008 Sep 15
TI  - A human dendritic cell subset receptive to the Venezuelan equine encephalitis 
      virus-derived replicon particle constitutively expresses IL-32.
PG  - 4010-8
AB  - Dendritic cells (DCs) are a diverse population with the capacity to respond to a 
      variety of pathogens. Because of their critical role in pathogenesis and 
      Ag-specific adaptive immune responses, DCs are the focus of extensive study and 
      incorporation into a variety of immunotherapeutic strategies. The diversity of DC 
      subsets imposes a substantial challenge to the successful development of DC-based 
      therapies, requiring identification of the involved subset(s) and the potential 
      roles each contributes to the immunologic responses. The recently developed and 
      promising Venezuelan equine encephalitis replicon particle (VRP) vector system 
      has conserved tropism for a subset of myeloid DCs. This immunotherapeutic vector 
      permits in situ targeting of DCs; however, it targets a restricted subset of DCs, 
      which are heretofore uncharacterized. Using a novel technique, we isolated 
      VRP-receptive and -nonreceptive populations from human monocyte-derived DCs. 
      Comparative gene expression analysis revealed significant differential gene 
      expression, supporting the existence of two distinct DC populations. Further 
      analysis identified constitutive expression of the proinflammatory cytokine IL-32 
      as a distinguishing characteristic of VRP-receptive DCs. IL-32 transcript was 
      exclusively expressed (>50 fold) in the VRP-receptive DC population relative to 
      the background level of expression in the nonreceptive population. The presence 
      of IL-32 transcript was accompanied by protein expression. These data are the 
      first to identify a subset of immature monocyte-derived DCs constitutively 
      expressing IL-32 and they provide insights into both DC biology and potential 
      mechanisms employed by this potent vector system.
FAU - Nishimoto, Kevin P
AU  - Nishimoto KP
AD  - Molecular Biology and Biochemistry, School of Biological Sciences, University of 
      California at Irvine, Irvine, CA 92697, USA.
FAU - Laust, Amanda K
AU  - Laust AK
FAU - Nelson, Edward L
AU  - Nelson EL
LA  - eng
GR  - P30 CA062203/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (IL32 protein, human)
RN  - 0 (Interleukins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Binding Sites/immunology
MH  - Cell Differentiation/genetics/immunology
MH  - Cell Membrane/immunology/metabolism/virology
MH  - Cell Separation
MH  - Dendritic Cells/cytology/*immunology/*virology
MH  - Encephalitis Virus, Venezuelan Equine/*immunology/pathogenicity
MH  - Gene Expression Profiling
MH  - Humans
MH  - Interleukins/*biosynthesis/*genetics/physiology
MH  - Monocytes/cytology/immunology/virology
MH  - Myeloid Progenitor Cells/cytology/immunology/virology
MH  - Oligonucleotide Array Sequence Analysis
MH  - RNA, Messenger/biosynthesis
MH  - Replicon/genetics/*immunology
MH  - Transcription, Genetic
MH  - Transduction, Genetic
PMC - PMC5629096
MID - NIHMS908952
COIS- Disclosures The authors have no financial conflicts of interest.
EDAT- 2008/09/05 09:00
MHDA- 2008/11/19 09:00
PMCR- 2017/10/05
CRDT- 2008/09/05 09:00
PHST- 2008/09/05 09:00 [pubmed]
PHST- 2008/11/19 09:00 [medline]
PHST- 2008/09/05 09:00 [entrez]
PHST- 2017/10/05 00:00 [pmc-release]
AID - 181/6/4010 [pii]
AID - 10.4049/jimmunol.181.6.4010 [doi]
PST - ppublish
SO  - J Immunol. 2008 Sep 15;181(6):4010-8. doi: 10.4049/jimmunol.181.6.4010.