PMID- 18768887
OWN - NLM
STAT- MEDLINE
DCOM- 20081118
LR  - 20190516
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Linking)
VI  - 181
IP  - 6
DP  - 2008 Sep 15
TI  - Hydrogen sulfide augments neutrophil migration through enhancement of adhesion 
      molecule expression and prevention of CXCR2 internalization: role of 
      ATP-sensitive potassium channels.
PG  - 4287-98
AB  - In this study, we have addressed the role of H(2)S in modulating neutrophil 
      migration in either innate (LPS-challenged naive mice) or adaptive (methylated 
      BSA (mBSA)-challenged immunized mice) immune responses. Treatment of mice with 
      H(2)S synthesis inhibitors, dl-propargylglycine (PAG) or beta-cyanoalanine, 
      reduced neutrophil migration induced by LPS or methylated BSA (mBSA) into the 
      peritoneal cavity and by mBSA into the femur/tibial joint of immunized mice. This 
      effect was associated with decreased leukocyte rolling, adhesion, and P-selectin 
      and ICAM-1 expression on endothelium. Predictably, treatment of animals with the 
      H(2)S donors, NaHS or Lawesson's reagent, enhanced these parameters. Moreover, 
      the NaHS enhancement of neutrophil migration was not observed in ICAM-1-deficient 
      mice. Neither PAG nor NaHS treatment changed LPS-induced CD18 expression on 
      neutrophils, nor did the LPS- and mBSA-induced release of neutrophil 
      chemoattractant mediators TNF-alpha, keratinocyte-derived chemokine, and LTB(4). 
      Furthermore, in vitro MIP-2-induced neutrophil chemotaxis was inhibited by PAG 
      and enhanced by NaHS treatments. Accordingly, MIP-2-induced CXCR2 internalization 
      was enhanced by PAG and inhibited by NaHS treatments. Moreover, NaHS prevented 
      MIP-2-induced CXCR2 desensitization. The PAG and NaHS effects correlated, 
      respectively, with the enhancement and inhibition of MIP-2-induced G 
      protein-coupled receptor kinase 2 expression. The effects of NaHS on neutrophil 
      migration both in vivo and in vitro, together with CXCR2 internalization and G 
      protein-coupled receptor kinase 2 expression were prevented by the ATP-sensitive 
      potassium (K(ATP)(+)) channel blocker, glybenclamide. Conversely, diazoxide, a 
      K(ATP)(+) channel opener, increased neutrophil migration in vivo. Together, our 
      data suggest that during the inflammatory response, H(2)S augments neutrophil 
      adhesion and locomotion, by a mechanism dependent on K(ATP)(+) channels.
FAU - Dal-Secco, Daniela
AU  - Dal-Secco D
AD  - Department of Pharmacology, School of Medicine of Ribeirao Preto, Ribeirao Preto, 
      Brazil.
FAU - Cunha, Thiago M
AU  - Cunha TM
FAU - Freitas, Andressa
AU  - Freitas A
FAU - Alves-Filho, Jose Carlos
AU  - Alves-Filho JC
FAU - Souto, Fabricio O
AU  - Souto FO
FAU - Fukada, Sandra Y
AU  - Fukada SY
FAU - Grespan, Renata
AU  - Grespan R
FAU - Alencar, Nylane M N
AU  - Alencar NM
FAU - Neto, Alberto F
AU  - Neto AF
FAU - Rossi, Marcos A
AU  - Rossi MA
FAU - Ferreira, Sergio H
AU  - Ferreira SH
FAU - Hothersall, John S
AU  - Hothersall JS
FAU - Cunha, Fernando Q
AU  - Cunha FQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (KATP Channels)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Receptors, Interleukin-8B)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - YY9FVM7NSN (Hydrogen Sulfide)
SB  - IM
MH  - Adjuvants, Immunologic/biosynthesis/*pharmacology
MH  - Animals
MH  - Cattle
MH  - Cell Adhesion Molecules/*biosynthesis/deficiency/genetics
MH  - Endocytosis/drug effects/*immunology
MH  - Gene Expression Regulation/drug effects/*immunology
MH  - Hydrogen Sulfide/metabolism/*pharmacology
MH  - Immunity, Innate/drug effects/genetics
MH  - KATP Channels/*physiology
MH  - Lipopolysaccharides/pharmacology
MH  - Male
MH  - Methylation/drug effects
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Neutrophil Infiltration/drug effects/*immunology
MH  - Receptors, Interleukin-8B/*antagonists & inhibitors/metabolism
MH  - Serum Albumin, Bovine/administration & dosage/immunology/metabolism
EDAT- 2008/09/05 09:00
MHDA- 2008/11/19 09:00
CRDT- 2008/09/05 09:00
PHST- 2008/09/05 09:00 [pubmed]
PHST- 2008/11/19 09:00 [medline]
PHST- 2008/09/05 09:00 [entrez]
AID - 181/6/4287 [pii]
AID - 10.4049/jimmunol.181.6.4287 [doi]
PST - ppublish
SO  - J Immunol. 2008 Sep 15;181(6):4287-98. doi: 10.4049/jimmunol.181.6.4287.