PMID- 18770746
OWN - NLM
STAT- MEDLINE
DCOM- 20080924
LR  - 20161021
IS  - 1934-9300 (Electronic)
IS  - 1934-9297 (Linking)
VI  - Chapter 8
DP  - 2001 May
TI  - Single-nucleotide sequence discrimination in situ using padlock probes.
PG  - Unit 8.8
LID - 10.1002/0471142956.cy0808s16 [doi]
AB  - Standard fluorescence in situ hybridization (FISH) techniques using cloned probes 
      are limited in their ability to distinguish between closely similar DNA sequences 
      because long hybridization probes are not detectably destabilized by single 
      mismatched base pairs. This problem has been addressed by using short 
      allele-specific oligonucleotide probes whose hybridization to target sequences is 
      more sensitive to mismatches. This revised and expanded unit presents protocols 
      for discrimination between closely similar DNA sequences in situ. The discussion 
      of probe synthesis has been greatly expanded and an Alternate Protocol 1 added 
      for enzymatic probe ligation at low probe concentration. A new Support Protocol 
      describes enzymatic probe synthesis.
FAU - Nilsson, M
AU  - Nilsson M
AD  - Uppsala University, Uppsala, Sweden.
FAU - Landegren, U
AU  - Landegren U
FAU - Antson, D O
AU  - Antson DO
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Curr Protoc Cytom
JT  - Current protocols in cytometry
JID - 100899351
RN  - 0 (DNA Probes)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Oligonucleotides)
RN  - 9007-49-2 (DNA)
RN  - EC 6.5.1.- (DNA Ligases)
SB  - IM
MH  - Alleles
MH  - Animals
MH  - DNA/analysis
MH  - DNA Ligases/metabolism
MH  - DNA Probes/*analysis
MH  - Fluorescent Dyes/chemistry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Molecular Probe Techniques
MH  - Nucleic Acid Hybridization
MH  - Oligonucleotides/analysis
EDAT- 2008/09/05 09:00
MHDA- 2008/09/25 09:00
CRDT- 2008/09/05 09:00
PHST- 2008/09/05 09:00 [pubmed]
PHST- 2008/09/25 09:00 [medline]
PHST- 2008/09/05 09:00 [entrez]
AID - 10.1002/0471142956.cy0808s16 [doi]
PST - ppublish
SO  - Curr Protoc Cytom. 2001 May;Chapter 8:Unit 8.8. doi: 
      10.1002/0471142956.cy0808s16.