PMID- 18770805
OWN - NLM
STAT- MEDLINE
DCOM- 20081216
LR  - 20211020
IS  - 1934-9300 (Electronic)
IS  - 1934-9297 (Linking)
VI  - Chapter 7
DP  - 2004 Nov
TI  - Differential staining of DNA and RNA.
PG  - Unit 7.3
LID - 10.1002/0471142956.cy0703s30 [doi]
AB  - Cell cycle analysis by means of differential staining of RNA and DNA permits 
      determination of RNA content, which in turn allows one to discriminate G(0) 
      versus G(1) cells and to detect cell differentiation. This unit presents two 
      protocols for differential staining, one using the metachromatic dye acridine 
      orange (AO) and the other a combination of pyronin Y (PY) and Hoechst 33342. Each 
      method has its advantages and limitations, and the Hoechst-PY method is not 
      applicable to single-laser instruments. A third protocol describes staining of 
      viable cells to identify/sort hematopoietic stem cells, with an alternative that 
      includes simultaneous immunostaining.
FAU - Darzynkiewicz, Zbigniew
AU  - Darzynkiewicz Z
AD  - New York Medical College, Valhalla, New York, USA.
FAU - Juan, Gloria
AU  - Juan G
FAU - Srour, Edward F
AU  - Srour EF
LA  - eng
GR  - R01 CA028704/CA/NCI NIH HHS/United States
GR  - R01 CA028704-26/CA/NCI NIH HHS/United States
GR  - R01 HL055716/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - Curr Protoc Cytom
JT  - Current protocols in cytometry
JID - 100899351
RN  - 0 (Benzimidazoles)
RN  - 63231-63-0 (RNA)
RN  - 9007-49-2 (DNA)
RN  - F30N4O6XVV (Acridine Orange)
RN  - P976261J69 (bisbenzimide ethoxide trihydrochloride)
RN  - W659G165T1 (Pyronine)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Benzimidazoles
MH  - *Cell Cycle
MH  - Cell Differentiation
MH  - Cytological Techniques/*methods
MH  - DNA/*analysis
MH  - Hematopoietic Stem Cells/cytology
MH  - Humans
MH  - Methods
MH  - Pyronine
MH  - RNA/*analysis
MH  - Staining and Labeling/*methods
EDAT- 2008/09/05 09:00
MHDA- 2008/12/17 09:00
CRDT- 2008/09/05 09:00
PHST- 2008/09/05 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/09/05 09:00 [entrez]
AID - 10.1002/0471142956.cy0703s30 [doi]
PST - ppublish
SO  - Curr Protoc Cytom. 2004 Nov;Chapter 7:Unit 7.3. doi: 
      10.1002/0471142956.cy0703s30.