PMID- 18771459
OWN - NLM
STAT- MEDLINE
DCOM- 20090413
LR  - 20160511
IS  - 1865-1674 (Print)
IS  - 1865-1674 (Linking)
VI  - 56
IP  - 1-2
DP  - 2009 Mar
TI  - Evaluation of the risk of PRRSV transmission via ingestion of muscle from 
      persistently infected pigs.
PG  - 1-8
LID - 10.1111/j.1865-1682.2008.01052.x [doi]
AB  - The objectives of this experiment were to determine how long porcine reproductive 
      and respiratory syndrome virus (PRRSV) could be detected in muscle tissues of 
      experimentally infected pigs and to evaluate the transmissibility of PRRSV to 
      pigs via ingestion of quantitative reverse-transcriptase polymerase chain 
      reaction (qRT-PCR)-positive muscle tissues. Serum, lymphoid tissues, and muscle 
      (M. longissimus dorsi) samples were collected from 135 pigs (89 PRRSV-inoculated 
      pigs and 46 negative control). Between 28 and 202 days post-inoculation, 13 of 89 
      (14.6%) muscle samples were positive by qRT-PCR. Among these 13, PRRSV was 
      isolated from four of the 13 corresponding serum samples and three of 13 lymphoid 
      tissue samples. In addition, infectious virus was detected in lymphoid tissue 
      homogenates of six of 13 pigs by intramuscular bioassay. Swine transmissibility 
      studies were performed by feeding thirteen 3-week-old PRRSV-naive pigs (recipient 
      pigs) qRT-PCR-positive muscle and then monitoring recipients for evidence of 
      PRRSV viremia by qRT-PCR. No transmission of PRRSV to recipient pigs via 
      consumption of muscle samples was observed. These data suggested that qRT-PCR 
      detected non-infectious PRRSV in pig meat and/or PRRSV is not highly 
      transmissible to susceptible pigs via consumption of PRRSV-contaminated meat.
FAU - Molina, R M
AU  - Molina RM
AD  - Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Iowa State 
      University, Ames, IA 50011 1250 , USA.
FAU - Nelson, E A
AU  - Nelson EA
FAU - Christopher-Hennings, J
AU  - Christopher-Hennings J
FAU - Hesse, R
AU  - Hesse R
FAU - Rowland, R R R
AU  - Rowland RR
FAU - Zimmerman, J J
AU  - Zimmerman JJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080902
PL  - Germany
TA  - Transbound Emerg Dis
JT  - Transboundary and emerging diseases
JID - 101319538
SB  - IM
MH  - Animals
MH  - Consumer Product Safety
MH  - Food Contamination/*analysis
MH  - Humans
MH  - Meat/*virology
MH  - Porcine Reproductive and Respiratory Syndrome/epidemiology/*transmission
MH  - Porcine respiratory and reproductive syndrome virus/*isolation & purification
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - *Risk Assessment
MH  - Swine
MH  - *Zoonoses
EDAT- 2008/09/06 09:00
MHDA- 2009/04/14 09:00
CRDT- 2008/09/06 09:00
PHST- 2008/09/06 09:00 [pubmed]
PHST- 2009/04/14 09:00 [medline]
PHST- 2008/09/06 09:00 [entrez]
AID - JVA1052 [pii]
AID - 10.1111/j.1865-1682.2008.01052.x [doi]
PST - ppublish
SO  - Transbound Emerg Dis. 2009 Mar;56(1-2):1-8. doi: 
      10.1111/j.1865-1682.2008.01052.x. Epub 2008 Sep 2.