PMID- 18772553
OWN - NLM
STAT- MEDLINE
DCOM- 20081112
LR  - 20190606
IS  - 0916-7250 (Print)
IS  - 0916-7250 (Linking)
VI  - 70
IP  - 8
DP  - 2008 Aug
TI  - Effect of the co-administration of vitamin C and vitamin E on tyrosine 
      hydroxylase and Nurr1 expression in the prenatal rat ventral mesencephalon.
PG  - 791-7
AB  - Tyrosine hydroxylase (TH) is the rate-limiting enzyme of dopamine (DA) 
      biosynthesis, which is up-regulated by vitamin C administration. Nurr1 gene is 
      highly expressed in brain and important for midbrain DAergic cell development and 
      survival. But, the role of vitamin C and/or vitamin E during Nurr1 expression is 
      yet to be known. Further, the synergistic effect of vitamins C and E on TH 
      expression has not yet been explored clinically. Therefore, we studied the 
      effects of single or co- administration of vitamin C (0.5 mM) and vitamin E (1 
      mM) for 72 hr, on both TH and Nurr1 expression in in vitro primary cultured 
      gestational days (GD) 13.5 rat ventral mesencephalon (VM) by Western blot and 
      immunocytochemistry. Our study revealed highest expressions of both TH and Nurr1 
      in the vitamin C + vitamin E treated group. TH expression was also increased in 
      the vitamin C treated group than that of the control group, but the vitamin E 
      treated group did not show any statistically significant effect. However, both 
      the vitamin C and the vitamin E treated groups revealed increased expression of 
      Nurr1 protein as compared with the control group. The present experimental data 
      suggest that vitamin C can up-regulate the protein expression of TH, but Nurr1 
      level was elevated either by vitamin C or by vitamin E administration. Further, 
      vitamin E acts synergistically with vitamin C to elevate TH and Nurr1 expression, 
      which is the most novel finding of our study and for the first time; we reported 
      this result, since there have been no published data on the synergistic effect of 
      both the antioxidant vitamins on TH and Nurr1 expression in VM. As the motor 
      function defect due to the progressive loss of DAergic neuron is the major reason 
      of Parkinsonism, therefore, the results of our study finally suggest the 
      effective role of vitamin C and vitamin E during early treatment of Parkinsonism.
FAU - Lee, Hae Young
AU  - Lee HY
AD  - Applied of Life Science Brain Korea 21, Gyeongsang National University, Jinju, 
      South Korea.
FAU - Naha, Nibedita
AU  - Naha N
FAU - Ullah, Najeeb
AU  - Ullah N
FAU - Jin, Guang Zhen
AU  - Jin GZ
FAU - Kong, Il Keun
AU  - Kong IK
FAU - Koh, Phil Ok
AU  - Koh PO
FAU - Seong, Hwan Hoo
AU  - Seong HH
FAU - Kim, Myeong Ok
AU  - Kim MO
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - J Vet Med Sci
JT  - The Journal of veterinary medical science
JID - 9105360
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nr4a2 protein, rat)
RN  - 0 (Nuclear Receptor Subfamily 4, Group A, Member 2)
RN  - 0 (Transcription Factors)
RN  - 1406-18-4 (Vitamin E)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - PQ6CK8PD0R (Ascorbic Acid)
SB  - IM
MH  - Animals
MH  - Ascorbic Acid/*pharmacology
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - DNA-Binding Proteins/drug effects/*metabolism
MH  - Female
MH  - Mesencephalon/drug effects/*embryology/metabolism
MH  - Nuclear Receptor Subfamily 4, Group A, Member 2
MH  - Pregnancy
MH  - Rats
MH  - Transcription Factors/drug effects/*metabolism
MH  - Tyrosine 3-Monooxygenase/drug effects/*metabolism
MH  - Vitamin E/*pharmacology
EDAT- 2008/09/06 09:00
MHDA- 2008/11/13 09:00
CRDT- 2008/09/06 09:00
PHST- 2008/09/06 09:00 [pubmed]
PHST- 2008/11/13 09:00 [medline]
PHST- 2008/09/06 09:00 [entrez]
AID - JST.JSTAGE/jvms/70.791 [pii]
AID - 10.1292/jvms.70.791 [doi]
PST - ppublish
SO  - J Vet Med Sci. 2008 Aug;70(8):791-7. doi: 10.1292/jvms.70.791.