PMID- 18772939
OWN - NLM
STAT- MEDLINE
DCOM- 20081202
LR  - 20080905
IS  - 0008-4166 (Print)
IS  - 0008-4166 (Linking)
VI  - 54
IP  - 9
DP  - 2008 Sep
TI  - An intracellular symbiont and other microbiota associated with field-collected 
      populations of sawflies (Hymenoptera: Symphyta).
PG  - 758-68
LID - 10.1139/w08-067 [doi]
AB  - Six species of sawfly (Hymenoptera: Symphyta) from four taxonomic families 
      (Agridae, Diprionidae, Pamphiliidae, and Tenthredinidae) were collected from 
      locations across Canada and surveyed for their associated microbiota. Total DNA 
      was extracted from individual insects, and polymerase chain reaction (PCR) was 
      used to amplify the conserved 16S rRNA gene from microbiota. Denaturing gradient 
      gel electrophoresis (DGGE) and restriction fragment length polymorphism (RFLP) 
      were undertaken to separate bacterial clones associated with the host insect. 
      Sequencing of the PCR-DGGE and PCR--RFLP products revealed a dominance of alpha- 
      and gamma-Proteobacteria, with most sequences showing high similarity to bacteria 
      previously identified from other insect species and environmental samples. 
      Additionally, a strain of the bacterial endosymbiont Wolbachia and a Wolbachia 
      bacteriophage were identified from the mountain ash sawfly (Pristiphora 
      geniculata).
FAU - Graham, Robert I
AU  - Graham RI
AD  - Population Ecology Group, Faculty of Forestry and Environmental Management, 
      University of New Brunswick, Fredericton, NB E3B6C2, Canada.
FAU - Zahner, Viviane
AU  - Zahner V
FAU - Lucarotti, Christopher J
AU  - Lucarotti CJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Can J Microbiol
JT  - Canadian journal of microbiology
JID - 0372707
SB  - IM
MH  - Animals
MH  - Bacteria/classification/genetics/*isolation & purification
MH  - *Bacterial Physiological Phenomena
MH  - Bacteriophages/genetics/*isolation & purification
MH  - Canada
MH  - Hymenoptera/*microbiology
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Polymorphism, Restriction Fragment Length
MH  - *Symbiosis
MH  - Wolbachia/genetics/isolation & purification/physiology/virology
EDAT- 2008/09/06 09:00
MHDA- 2008/12/17 09:00
CRDT- 2008/09/06 09:00
PHST- 2008/09/06 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/09/06 09:00 [entrez]
AID - w08-067 [pii]
AID - 10.1139/w08-067 [doi]
PST - ppublish
SO  - Can J Microbiol. 2008 Sep;54(9):758-68. doi: 10.1139/w08-067.