PMID- 18773968
OWN - NLM
STAT- MEDLINE
DCOM- 20090204
LR  - 20081125
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 657
IP  - 2
DP  - 2008 Dec 8
TI  - Induction and removal of DNA damage in blood leukocytes of patients with type 2 
      diabetes mellitus undergoing hemodialysis.
PG  - 111-5
LID - 10.1016/j.mrgentox.2008.08.004 [doi]
AB  - Type 2 diabetes mellitus (T2DM) is associated with a high production of reactive 
      oxygen species, which may cause oxidative DNA damage. High levels of genomic 
      damage have been associated with renal failure and hemodialysis. However, no 
      information is available in the literature concerning the levels of DNA damage in 
      T2DM individuals who are dependent on hemodialysis. This study used the comet 
      assay to assess the levels of DNA damage before, immediately after and 48 h after 
      the hemodialysis session in 25 patients with T2DM and in a group of 20 healthy 
      individuals, selected according to mean age, sex and smoking habit. Our results 
      showed increased levels of DNA damage in hemodialysis-dependent T2DM individuals 
      (12.36+/-8.04) when compared with healthy individuals (7.35+/-7.41) (p=0.014). 
      Damage levels increased immediately after the hemodialysis session 
      (19.76+/-12.40) (p=0.04), which suggests a possible action of pro-oxidative 
      factors related to the therapy, with a genotoxic effect on cells. Results 
      obtained 48 h after hemodialysis (6.44+/-5.99) evidenced damage removal 
      (p=0.001), which may be suggestive of DNA repair.
FAU - Bagatini, Pamela Brambilla
AU  - Bagatini PB
AD  - Health Science Institute, Feevale University, RS 239, 2755, CEP 93352-000, Novo 
      Hamburgo, RS, Brazil.
FAU - Palazzo, Roberta Passos
AU  - Palazzo RP
FAU - Rodrigues, Manoela Tressoldi
AU  - Rodrigues MT
FAU - Costa, Cynthia Hernandes
AU  - Costa CH
FAU - Maluf, Sharbel Weidner
AU  - Maluf SW
LA  - eng
PT  - Journal Article
DEP - 20080819
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
SB  - IM
MH  - Aged
MH  - Comet Assay
MH  - *DNA Damage
MH  - DNA Repair
MH  - Diabetes Mellitus, Type 2/*metabolism/*therapy
MH  - Female
MH  - Humans
MH  - Leukocytes/*drug effects
MH  - Male
MH  - Middle Aged
MH  - Oxidative Stress
MH  - Renal Dialysis
EDAT- 2008/09/09 09:00
MHDA- 2009/02/05 09:00
CRDT- 2008/09/09 09:00
PHST- 2008/06/26 00:00 [received]
PHST- 2008/08/06 00:00 [revised]
PHST- 2008/08/07 00:00 [accepted]
PHST- 2008/09/09 09:00 [pubmed]
PHST- 2009/02/05 09:00 [medline]
PHST- 2008/09/09 09:00 [entrez]
AID - S1383-5718(08)00235-0 [pii]
AID - 10.1016/j.mrgentox.2008.08.004 [doi]
PST - ppublish
SO  - Mutat Res. 2008 Dec 8;657(2):111-5. doi: 10.1016/j.mrgentox.2008.08.004. Epub 
      2008 Aug 19.